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• W2417271597 endingPage "3988" @default.
• W2417271597 startingPage "3978" @default.
• W2417271597 abstract "The acetoacetate decarboxylase-like superfamily (ADCSF) is a group of ~4000 enzymes that, until recently, was thought to be homogeneous in terms of the reaction catalyzed. Bioinformatic analysis shows that the ADCSF consists of up to seven families that differ primarily in their active site architectures. The soil-dwelling bacterium Streptomyces bingchenggensis BCW-1 produces an ADCSF enzyme of unknown function that shares a low level of sequence identity (~20%) with known acetoacetate decarboxylases (ADCs). This enzyme, Sbi00515, belongs to the MppR-like family of the ADCSF because of its similarity to the mannopeptimycin biosynthetic protein MppR from Streptomyces hygroscopicus. Herein, we present steady state kinetic data that show Sbi00515 does not catalyze the decarboxylation of any α- or β-keto acid tested. Rather, we show that Sbi00515 catalyzes the condensation of pyruvate with a number of aldehydes, followed by dehydration of the presumed aldol intermediate. Thus, Sbi00515 is a pyruvate aldolase-dehydratase and not an acetoacetate decarboxylase. We have also determined the X-ray crystal structures of Sbi00515 in complexes with formate and pyruvate. The structures show that the overall fold of Sbi00515 is nearly identical to those of both ADC and MppR. The pyruvate complex is trapped as the Schiff base, providing evidence that the Schiff base chemistry that drives the acetoacetate decarboxylases has been co-opted to perform a new function, and that this core chemistry may be conserved across the superfamily. The structures also suggest possible catalytic roles for several active site residues." @default.
• W2417271597 created "2016-06-24" @default.
• W2417271597 creator A5004875104 @default.
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• W2417271597 date "2015-06-03" @default.
• W2417271597 modified "2023-09-23" @default.
• W2417271597 title "Sbi00515, a Protein of Unknown Function from <i>Streptomyces bingchenggensis</i>, Highlights the Functional Versatility of the Acetoacetate Decarboxylase Scaffold" @default.
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• W2417271597 doi "https://doi.org/10.1021/acs.biochem.5b00483" @default.
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