FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2417362980> ?p ?o ?g. }

• W2417362980 endingPage "70" @default.
• W2417362980 startingPage "64" @default.
• W2417362980 abstract "Abstract The activity of delta-aminolevulinic acid (ALA) dehydratase, an enzyme involved in heme biosynthesis, has been shown to increase in Friend virus-transformed murine erythroleukemia (MEL) cells during erythroid differentiation. In this study, the nature of the increase in ALA dehydratase activity in MEL cells was examined using a monospecific antibody directed to the enzyme. A sevenfold increase in ALA dehydratase activity was observed after cells had been treated with 1.5% Me2SO for 5 days. Ouchterlony double immunodiffusion analysis showed that lysates from untreated and Me2SO-treated MEL cells formed a single precipitin line with rabbit IgG directed to the normal mouse liver ALA dehydratase. A single arc of identity was also observed with the lysates from normal mouse erythrocytes, spleen, liver, and lysates from both uninduced and induced MEL cells. Rocket immunoelectrophoresis demonstrated that lysates from both uninduced and induced cells formed rockets with the IgG and that the peak height of the rocket was proportional to the ALA dehydratase activity applied. The slope of linear plots of rocket peak heights v ALA dehydratase activity was identical for lysates from uninduced and Me2SO-induced cells. Succinylacetone, a potent inhibitor of ALA dehydratase, was shown to markedly inhibit the activity of the enzyme, but did not interfere with the synthesis of ALA dehydratase induced by Me2SO treatment. Me2SO- induced increases in ALA dehydratase activity and the enzyme protein were both blocked by the simultaneous treatment of cells with 5-bromo- 2′-deoxyuridine (BrdU). BrdU-mediated repression of ALA dehydratase was partially overcome by treating the cells with thymidine. These data demonstrate that increased ALA dehydratase activity in MEL cells undergoing erythroid differentiation after Me2SO treatment is due to de novo synthesis of the same enzyme protein present in uninduced MEL cells as well as in normal erythrocytes. This represents the first direct demonstration of an increase in a heme biosynthetic pathway enzyme protein in erythroid cells undergoing differentiation." @default.
• W2417362980 created "2016-06-24" @default.
• W2417362980 creator A5074623028 @default.
• W2417362980 creator A5086841844 @default.
• W2417362980 date "1984-07-01" @default.
• W2417362980 modified "2023-10-15" @default.
• W2417362980 title "Induction of delta-aminolevulinic acid dehydratase in mouse Friend virus-transformed erythroleukemia cells during erythroid differentiation" @default.
• W2417362980 cites W1005085010 @default.
• W2417362980 cites W1547928236 @default.
• W2417362980 cites W1577480508 @default.
• W2417362980 cites W161706433 @default.
• W2417362980 cites W1625660792 @default.
• W2417362980 cites W1974333347 @default.
• W2417362980 cites W1983074084 @default.
• W2417362980 cites W1987877328 @default.
• W2417362980 cites W1990986783 @default.
• W2417362980 cites W2010667027 @default.
• W2417362980 cites W2029665225 @default.
• W2417362980 cites W2081959583 @default.
• W2417362980 cites W2400418602 @default.
• W2417362980 cites W73019834 @default.
• W2417362980 cites W95213172 @default.
• W2417362980 cites W2234565176 @default.
• W2417362980 doi "https://doi.org/10.1182/blood.v64.1.64.64" @default.
• W2417362980 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6587918" @default.
• W2417362980 hasPublicationYear "1984" @default.
• W2417362980 type Work @default.
• W2417362980 sameAs 2417362980 @default.
• W2417362980 citedByCount "16" @default.
• W2417362980 crossrefType "journal-article" @default.
• W2417362980 hasAuthorship W2417362980A5074623028 @default.
• W2417362980 hasAuthorship W2417362980A5086841844 @default.
• W2417362980 hasBestOaLocation W24173629801 @default.
• W2417362980 hasConcept C153911025 @default.
• W2417362980 hasConcept C159654299 @default.
• W2417362980 hasConcept C181199279 @default.
• W2417362980 hasConcept C185592680 @default.
• W2417362980 hasConcept C203014093 @default.
• W2417362980 hasConcept C2780931953 @default.
• W2417362980 hasConcept C2781374117 @default.
• W2417362980 hasConcept C28212737 @default.
• W2417362980 hasConcept C44538786 @default.
• W2417362980 hasConcept C54355233 @default.
• W2417362980 hasConcept C55493867 @default.
• W2417362980 hasConcept C80020023 @default.
• W2417362980 hasConcept C81885089 @default.
• W2417362980 hasConcept C86803240 @default.
• W2417362980 hasConcept C91523082 @default.
• W2417362980 hasConceptScore W2417362980C153911025 @default.
• W2417362980 hasConceptScore W2417362980C159654299 @default.
• W2417362980 hasConceptScore W2417362980C181199279 @default.
• W2417362980 hasConceptScore W2417362980C185592680 @default.
• W2417362980 hasConceptScore W2417362980C203014093 @default.
• W2417362980 hasConceptScore W2417362980C2780931953 @default.
• W2417362980 hasConceptScore W2417362980C2781374117 @default.
• W2417362980 hasConceptScore W2417362980C28212737 @default.
• W2417362980 hasConceptScore W2417362980C44538786 @default.
• W2417362980 hasConceptScore W2417362980C54355233 @default.
• W2417362980 hasConceptScore W2417362980C55493867 @default.
• W2417362980 hasConceptScore W2417362980C80020023 @default.
• W2417362980 hasConceptScore W2417362980C81885089 @default.
• W2417362980 hasConceptScore W2417362980C86803240 @default.
• W2417362980 hasConceptScore W2417362980C91523082 @default.
• W2417362980 hasIssue "1" @default.
• W2417362980 hasLocation W24173629801 @default.
• W2417362980 hasOpenAccess W2417362980 @default.
• W2417362980 hasPrimaryLocation W24173629801 @default.
• W2417362980 hasRelatedWork W1699920965 @default.
• W2417362980 hasRelatedWork W1918045784 @default.
• W2417362980 hasRelatedWork W2038908275 @default.
• W2417362980 hasRelatedWork W2052141035 @default.
• W2417362980 hasRelatedWork W2055370189 @default.
• W2417362980 hasRelatedWork W2073134219 @default.
• W2417362980 hasRelatedWork W2342448571 @default.
• W2417362980 hasRelatedWork W2400418602 @default.
• W2417362980 hasRelatedWork W2408911902 @default.
• W2417362980 hasRelatedWork W958171835 @default.
• W2417362980 hasVolume "64" @default.
• W2417362980 isParatext "false" @default.
• W2417362980 isRetracted "false" @default.
• W2417362980 magId "2417362980" @default.
• W2417362980 workType "article" @default.