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- W2417496633 abstract "Microtubule-severing enzymes are a novel class of microtubule regulators. They are enzymes from the ATPases associated with various cellular activities family (AAA+) that utilize ATP to cut microtubules into smaller filaments. Discovered over 20 years ago, there are still many open questions about severing enzymes. Both cellular and biochemical studies need to be pursued to fully understand how these enzymes function mechanistically in the cell. Here, we present methods to express, purify, and test the biophysical nature of these proteins in vitro to begin to address the biochemical and biophysical mechanisms of this important and novel group of microtubule destabilizers." @default.
- W2417496633 created "2016-06-24" @default.
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- W2417496633 date "2013-01-01" @default.
- W2417496633 modified "2023-10-01" @default.
- W2417496633 title "Purification and Biophysical Analysis of Microtubule-Severing Enzymes In Vitro" @default.
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- W2417496633 doi "https://doi.org/10.1016/b978-0-12-407757-7.00013-x" @default.
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