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• W2417734563 abstract "Recombinant DNA technology has provided a windfall of information about the structure and function of the gene. This information has enabled the basic researcher to examine the mechanisms controlling prokaryotic and eukaryotic gene expression in ways that were not possible with conventional genetic and biochemical approaches. The fruits of this research have already been applied to the overproduction of commercially important peptides by the biotechnology industry. However, before gene expression can be manipulated in a predictable fashion, it is necessary to thoroughly understand those regulatory genetic elements known as promoters. Because of the cis-acting nature of promoters, the study of promoters poses unique problems for the investigator. For example, mutations affecting the structure and function of a promoter cannot be suppressed or complemented with wild-type alleles. Therefore, promoters for genes that are critical to the cell or whose product cannot be readily measured are difficult to study. If, however, a gene fusion is constructed between the promoter of interest and a marker or recorder gene, the promoter is analyzed independently of the gene it controls. This reasoning leads to the design and development of the promoter-probe vectors. This chapter explains a variety of plasmids that are used to analyze promoters and terminators in Saccharomyces cerevisiae. These vectors permit regulatory elements to be examined under normal physiological conditions. In this way, the direct effects of nucleotide sequences and ancillary components involved in gene regulation are observed and quantified." @default.
• W2417734563 created "2016-06-24" @default.
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• W2417734563 date "1988-01-01" @default.
• W2417734563 modified "2023-09-27" @default.
• W2417734563 title "Plasmid Vectors for the Analysis of Regulatory Sequences in Yeast" @default.
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• W2417734563 doi "https://doi.org/10.1016/b978-0-409-90042-2.50026-x" @default.
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