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- W2417754549 abstract "Standard immunogold-labeling methods in transmission electron microscopy (TEM) are unable to locate immunogold particles in the depth direction. This inability does not only concern bulky whole mounts, but also sections. A partial solution to the problem is stereo inspection. However, three-dimensional reconstruction of immunogold-labeled structures, that is, immuno-electron tomography (IET), is a correct solution for this inconsistency. Striking improvement in resolution is achieved: the 1.4-nm immunogold particles are shown in IET that are not detected in the original tilt series. IET is not restricted to laboratories with advanced medium- or high-voltage TEM and super-computing facilities; the methods we have developed for whole-mounted chromosomes and also for whole-mounted cytoskeleton of fibroblasts work remarkably well with ordinary 80-kV TEMs equipped with a goniometer to collect tilt series for IET on film. In addition, free programs are available to produce three-dimensional reconstructions even without high-performance computers. These improvements make it possible to many laboratories without modern facilities to perform IET reconstruction with standard TEM apparatus." @default.
- W2417754549 created "2016-06-24" @default.
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- W2417754549 date "2007-01-01" @default.
- W2417754549 modified "2023-10-16" @default.
- W2417754549 title "Whole-Mount Immunoelectron Tomography of Chromosomes and Cells" @default.
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- W2417754549 doi "https://doi.org/10.1007/978-1-59745-294-6_19" @default.
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