Matches in SemOpenAlex for { <https://semopenalex.org/work/W2417963496> ?p ?o ?g. }
- W2417963496 endingPage "1337" @default.
- W2417963496 startingPage "1331" @default.
- W2417963496 abstract "Tyrosinases, copper-containing monooxygenases, are widely used enzymes for industrial, medical, and environmental applications. We report the first functional surface display of Bacillus megaterium tyrosinase on Bacillus subtilis spores using CotE as an anchor protein. Flow Cytometry was used to verify surface expression of tyrosinase on the purified spores. Moreover, tyrosinase activity of the displayed enzyme on B. subtilis spores was monitored in the presence of L-tyrosine (substrate) and CuSO4 (inducer). The stability of the spore-displayed tyrosinase was then evaluated after 15 days maintenance of the spores at room temperature, and no significant decrease in the enzyme activity was observed. In addition, the tyrosinase-expressing spores could be repeatedly used with 62% retained enzymatic activity after six times washing with Tris-HCl buffer. This genetically immobilized tyrosinase on the spores would make a new advance in industrial, medical, and environmental applications." @default.
- W2417963496 created "2016-06-24" @default.
- W2417963496 creator A5002739553 @default.
- W2417963496 creator A5026083872 @default.
- W2417963496 creator A5052315121 @default.
- W2417963496 creator A5054363871 @default.
- W2417963496 creator A5072604486 @default.
- W2417963496 creator A5084664076 @default.
- W2417963496 date "2016-06-09" @default.
- W2417963496 modified "2023-10-05" @default.
- W2417963496 title "Surface display of bacterial tyrosinase on spores of<i>Bacillus subtilis</i>using CotE as an anchor protein" @default.
- W2417963496 cites W1586033428 @default.
- W2417963496 cites W1966272449 @default.
- W2417963496 cites W1971218701 @default.
- W2417963496 cites W1972781731 @default.
- W2417963496 cites W1974781567 @default.
- W2417963496 cites W1998164896 @default.
- W2417963496 cites W2005523602 @default.
- W2417963496 cites W2010510622 @default.
- W2417963496 cites W2010515322 @default.
- W2417963496 cites W2026196512 @default.
- W2417963496 cites W2030408550 @default.
- W2417963496 cites W2032613666 @default.
- W2417963496 cites W2046999075 @default.
- W2417963496 cites W2050466522 @default.
- W2417963496 cites W2051069777 @default.
- W2417963496 cites W2064048621 @default.
- W2417963496 cites W2081274074 @default.
- W2417963496 cites W2083304588 @default.
- W2417963496 cites W2105239802 @default.
- W2417963496 cites W2114529732 @default.
- W2417963496 cites W2121644765 @default.
- W2417963496 cites W2125637425 @default.
- W2417963496 cites W2126353147 @default.
- W2417963496 cites W2140775467 @default.
- W2417963496 cites W2143970189 @default.
- W2417963496 cites W2150230381 @default.
- W2417963496 cites W2151641754 @default.
- W2417963496 cites W2321641551 @default.
- W2417963496 cites W360238216 @default.
- W2417963496 cites W4241731181 @default.
- W2417963496 doi "https://doi.org/10.1002/jobm.201600203" @default.
- W2417963496 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27281458" @default.
- W2417963496 hasPublicationYear "2016" @default.
- W2417963496 type Work @default.
- W2417963496 sameAs 2417963496 @default.
- W2417963496 citedByCount "20" @default.
- W2417963496 countsByYear W24179634962017 @default.
- W2417963496 countsByYear W24179634962018 @default.
- W2417963496 countsByYear W24179634962019 @default.
- W2417963496 countsByYear W24179634962020 @default.
- W2417963496 countsByYear W24179634962021 @default.
- W2417963496 countsByYear W24179634962022 @default.
- W2417963496 countsByYear W24179634962023 @default.
- W2417963496 crossrefType "journal-article" @default.
- W2417963496 hasAuthorship W2417963496A5002739553 @default.
- W2417963496 hasAuthorship W2417963496A5026083872 @default.
- W2417963496 hasAuthorship W2417963496A5052315121 @default.
- W2417963496 hasAuthorship W2417963496A5054363871 @default.
- W2417963496 hasAuthorship W2417963496A5072604486 @default.
- W2417963496 hasAuthorship W2417963496A5084664076 @default.
- W2417963496 hasConcept C174618031 @default.
- W2417963496 hasConcept C181199279 @default.
- W2417963496 hasConcept C185592680 @default.
- W2417963496 hasConcept C18903297 @default.
- W2417963496 hasConcept C2777272437 @default.
- W2417963496 hasConcept C2777289219 @default.
- W2417963496 hasConcept C2780989563 @default.
- W2417963496 hasConcept C523546767 @default.
- W2417963496 hasConcept C54355233 @default.
- W2417963496 hasConcept C55493867 @default.
- W2417963496 hasConcept C86803240 @default.
- W2417963496 hasConcept C87554066 @default.
- W2417963496 hasConcept C89423630 @default.
- W2417963496 hasConceptScore W2417963496C174618031 @default.
- W2417963496 hasConceptScore W2417963496C181199279 @default.
- W2417963496 hasConceptScore W2417963496C185592680 @default.
- W2417963496 hasConceptScore W2417963496C18903297 @default.
- W2417963496 hasConceptScore W2417963496C2777272437 @default.
- W2417963496 hasConceptScore W2417963496C2777289219 @default.
- W2417963496 hasConceptScore W2417963496C2780989563 @default.
- W2417963496 hasConceptScore W2417963496C523546767 @default.
- W2417963496 hasConceptScore W2417963496C54355233 @default.
- W2417963496 hasConceptScore W2417963496C55493867 @default.
- W2417963496 hasConceptScore W2417963496C86803240 @default.
- W2417963496 hasConceptScore W2417963496C87554066 @default.
- W2417963496 hasConceptScore W2417963496C89423630 @default.
- W2417963496 hasFunder F4320321292 @default.
- W2417963496 hasFunder F4320322349 @default.
- W2417963496 hasFunder F4320324152 @default.
- W2417963496 hasIssue "12" @default.
- W2417963496 hasLocation W24179634961 @default.
- W2417963496 hasLocation W24179634962 @default.
- W2417963496 hasOpenAccess W2417963496 @default.
- W2417963496 hasPrimaryLocation W24179634961 @default.
- W2417963496 hasRelatedWork W1504924446 @default.
- W2417963496 hasRelatedWork W1565639787 @default.
- W2417963496 hasRelatedWork W1827908195 @default.