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- W2418063463 abstract "The aim of this study was to perform the validation of nested RT-PCR method for detection of measles virus genome in laboratory of Virology Department (NIZP-PZH) conditions. The PCR reactions were made with specific primers for nucleoprotein gene. The validation of nested RT-PCR assay includes determination of limit of detection (LOD), specificity, intra- and inter-assay precision and influence of materials from clinical samples on the PCR reaction. The detection limit of nested RT-PCR was 255-510 RNA MeV/ml. The obtained results reveal high precision and lack of substances present in clinical samples influence on the PCR reaction. The application of nested RT-PCR assay for viral genomes other than MeV gave negative results. The obtained results indicated that the nested RT-PCR fulfilled validation criteria and can be used for routine laboratory diagnostics of measles virus." @default.
- W2418063463 created "2016-06-24" @default.
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- W2418063463 date "2008-01-01" @default.
- W2418063463 modified "2023-09-25" @default.
- W2418063463 title "[Validation of nested RT-PCR method for detection of measles virus in clinical samples]." @default.
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