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- W2418112757 abstract "A fundamental process in DNA replication is the disentangling of the two parental strands by DNA topoisomerases. In this chapter, I detail the topological analysis of plasmid replication intermediates using two-dimensional (2D) agarose gels. The method can resolve replication intermediates according to mass and topology, and can resolve unlinked monomeric circles from catenated dimers of varying topology. The method has been used, alone or in combination with a procedure for purifying covalent protein-DNA complexes, to analyse the effect oftopoisomerase inhibitors on the topology of replication intermediates, to map the location of drug-stabilized topoisomerase cleavage complexes with respect to replication forks and to detect the breakage and repair of replication forks following collision with cleavage complexes. Other applications include the detection of knots that form independently of, or concomitantly with, DNA replication." @default.
- W2418112757 created "2016-06-24" @default.
- W2418112757 creator A5007655086 @default.
- W2418112757 date "2009-01-01" @default.
- W2418112757 modified "2023-09-26" @default.
- W2418112757 title "Topological Analysis of Plasmid DNA Replication Intermediates Using Two-Dimensional Agarose Gels" @default.
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- W2418112757 doi "https://doi.org/10.1007/978-1-60327-815-7_8" @default.
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