Matches in SemOpenAlex for { <https://semopenalex.org/work/W2418167380> ?p ?o ?g. }
Showing items 1 to 68 of
68
with 100 items per page.
- W2418167380 endingPage "54" @default.
- W2418167380 startingPage "345" @default.
- W2418167380 abstract "The assembly kinetics of Xenopus wild type lamin A and 7 mutants were analyzed by the microinjection of renatured 5-iodoacetamidofluorescein (5-IAF)-labeled protein into mouse 3T3 cells. This experimental system has the advantage that the masking of the mutant phenotype by the formation of heterooligomeric complexes with newly synthesized lamins of the microinjected cell is negligibly small. The wild type protein and all mutants containing a nuclear localization signal were transported within 10 min into the nucleus. The wild type protein exhibited a strong lamina fluorescence 30 min after microinjection, whereas mutant molecules showed a delayed but complete, a delayed and incomplete, or no lamina assembly at all. The lamin A mutant lacking the carboxy-terminal cysteine of the CxxM-motif exhibited a delayed but complete assembly, whereas previously performed transfection experiments demonstrated no significant interaction of this mutant wih the lamina. According to present knowledge this phenotype in transfected cells can be explained by an overexpression of this mutant. We have identified an additional domain in the carboxy-terminal tail of lamin A that promotes its assembly into the lamina. In vitro this domain is required for the chromatin binding of lamin A. Lamin A molecules lacking the non-helical amino-terminal head domain showed no significant lamina staining, whereas point mutations in conserved regions of the alpha helix resulted in an incomplete assembly." @default.
- W2418167380 created "2016-06-24" @default.
- W2418167380 creator A5035350837 @default.
- W2418167380 creator A5053053716 @default.
- W2418167380 date "1995-12-01" @default.
- W2418167380 modified "2023-09-23" @default.
- W2418167380 title "In vivo assembly kinetics of fluorescently labeled Xenopus lamin A mutants." @default.
- W2418167380 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8690014" @default.
- W2418167380 hasPublicationYear "1995" @default.
- W2418167380 type Work @default.
- W2418167380 sameAs 2418167380 @default.
- W2418167380 citedByCount "3" @default.
- W2418167380 crossrefType "journal-article" @default.
- W2418167380 hasAuthorship W2418167380A5035350837 @default.
- W2418167380 hasAuthorship W2418167380A5053053716 @default.
- W2418167380 hasConcept C104317684 @default.
- W2418167380 hasConcept C143065580 @default.
- W2418167380 hasConcept C153911025 @default.
- W2418167380 hasConcept C179093185 @default.
- W2418167380 hasConcept C185592680 @default.
- W2418167380 hasConcept C207583985 @default.
- W2418167380 hasConcept C2779535977 @default.
- W2418167380 hasConcept C2780723820 @default.
- W2418167380 hasConcept C29512474 @default.
- W2418167380 hasConcept C54009773 @default.
- W2418167380 hasConcept C55493867 @default.
- W2418167380 hasConcept C62525196 @default.
- W2418167380 hasConcept C86339819 @default.
- W2418167380 hasConcept C86803240 @default.
- W2418167380 hasConcept C94912109 @default.
- W2418167380 hasConcept C95444343 @default.
- W2418167380 hasConceptScore W2418167380C104317684 @default.
- W2418167380 hasConceptScore W2418167380C143065580 @default.
- W2418167380 hasConceptScore W2418167380C153911025 @default.
- W2418167380 hasConceptScore W2418167380C179093185 @default.
- W2418167380 hasConceptScore W2418167380C185592680 @default.
- W2418167380 hasConceptScore W2418167380C207583985 @default.
- W2418167380 hasConceptScore W2418167380C2779535977 @default.
- W2418167380 hasConceptScore W2418167380C2780723820 @default.
- W2418167380 hasConceptScore W2418167380C29512474 @default.
- W2418167380 hasConceptScore W2418167380C54009773 @default.
- W2418167380 hasConceptScore W2418167380C55493867 @default.
- W2418167380 hasConceptScore W2418167380C62525196 @default.
- W2418167380 hasConceptScore W2418167380C86339819 @default.
- W2418167380 hasConceptScore W2418167380C86803240 @default.
- W2418167380 hasConceptScore W2418167380C94912109 @default.
- W2418167380 hasConceptScore W2418167380C95444343 @default.
- W2418167380 hasIssue "4" @default.
- W2418167380 hasLocation W24181673801 @default.
- W2418167380 hasOpenAccess W2418167380 @default.
- W2418167380 hasPrimaryLocation W24181673801 @default.
- W2418167380 hasRelatedWork W1489293129 @default.
- W2418167380 hasRelatedWork W1581525453 @default.
- W2418167380 hasRelatedWork W2001272941 @default.
- W2418167380 hasRelatedWork W2009464217 @default.
- W2418167380 hasRelatedWork W2011804485 @default.
- W2418167380 hasRelatedWork W204208616 @default.
- W2418167380 hasRelatedWork W2094615509 @default.
- W2418167380 hasRelatedWork W2367177189 @default.
- W2418167380 hasRelatedWork W2384752929 @default.
- W2418167380 hasRelatedWork W2418167380 @default.
- W2418167380 hasVolume "68" @default.
- W2418167380 isParatext "false" @default.
- W2418167380 isRetracted "false" @default.
- W2418167380 magId "2418167380" @default.
- W2418167380 workType "article" @default.