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- W2418263120 abstract "To establish mouse lens epithelial cell lines with the genotype of Hsf4-/-.The expended mouse lens epithelial cells, which were generated from P6 Hsf4-deficient mouse lens epithelia, were immortalized with SV40-T-antigen and named MLEC/Hsf4-/- cell. The expression of alpha A-crystallin was immunoblotted. Hsf4b cDNA was reconstituted by transiently transfection.The SV40-immortalized cells were in adherent growth mode with spindle morphology, pseudopodia, clear nuclear boundary membrane and cytoplasm translucent. Immunoblotting results indicated that the lens biomarker protein alpha A-crystallin was expressed in MLEC/Hsf4-/- cells. Reconstitution of Hsf4b into MLEC/Hsf4-/- cells upregulated the expression of Hsp25.The SV40-immortalized MLEC/Hsf4-/- cells have the lens epithelial characteristics and could be used as a tool for studying the signal transduction in vitro." @default.
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- W2418263120 date "2013-11-01" @default.
- W2418263120 modified "2023-09-23" @default.
- W2418263120 title "[Establishment of mouse lens epithelial cell lines with the genotype of Hsf4-/-]." @default.
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