Matches in SemOpenAlex for { <https://semopenalex.org/work/W2418515572> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W2418515572 endingPage "102" @default.
- W2418515572 startingPage "99" @default.
- W2418515572 abstract "Purpose To establish a simple and convenient method for the culture of human umbilical vein endothelial cells (HUVECs) and study its characterization in vitro. Methods Human Umbilical cord was isolated and digested by collagenase type I,and then it was cultured in 1.5% geltain coated dish with 10% fetal bovine serum(FBS)，heparin sodium andβ-endothelial cell growth factor(β-ECGF) in human endothelial basal growth medium．HUVECs were identified by anti-human factor Ⅷ related antigen and CD31 immunohistochemical staining． Results We could successfully culture HUVECs by using this method. HUVECs attached the dish in 24 hours and the confluence was seen in 5 days. HUVECs were generally positive for anti-human factor Ⅷ related antigen and CD31． Conclusion It is a fast and effective method to successfully culture purified HUVECs in which collagenase type I was used to digest HUVECs with glass tube connected to T-tube, human endothelial basal growth medium containing 10% FBS,heparin sodium and β-ECGF in 1.5% geltain coated dish." @default.
- W2418515572 created "2016-06-24" @default.
- W2418515572 creator A5007382912 @default.
- W2418515572 creator A5043226608 @default.
- W2418515572 creator A5061314408 @default.
- W2418515572 creator A5074850486 @default.
- W2418515572 creator A5075848637 @default.
- W2418515572 creator A5075890835 @default.
- W2418515572 date "2010-11-01" @default.
- W2418515572 modified "2023-09-24" @default.
- W2418515572 title "An improved method of culturing human umbilical vein endothelial cells and its characterization" @default.
- W2418515572 doi "https://doi.org/10.3969/g.issn.1000-4432.2010.02.010" @default.
- W2418515572 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21186489" @default.
- W2418515572 hasPublicationYear "2010" @default.
- W2418515572 type Work @default.
- W2418515572 sameAs 2418515572 @default.
- W2418515572 citedByCount "0" @default.
- W2418515572 crossrefType "journal-article" @default.
- W2418515572 hasAuthorship W2418515572A5007382912 @default.
- W2418515572 hasAuthorship W2418515572A5043226608 @default.
- W2418515572 hasAuthorship W2418515572A5061314408 @default.
- W2418515572 hasAuthorship W2418515572A5074850486 @default.
- W2418515572 hasAuthorship W2418515572A5075848637 @default.
- W2418515572 hasAuthorship W2418515572A5075890835 @default.
- W2418515572 hasConcept C123012128 @default.
- W2418515572 hasConcept C153911025 @default.
- W2418515572 hasConcept C16685009 @default.
- W2418515572 hasConcept C181199279 @default.
- W2418515572 hasConcept C185592680 @default.
- W2418515572 hasConcept C202751555 @default.
- W2418515572 hasConcept C203014093 @default.
- W2418515572 hasConcept C2776955114 @default.
- W2418515572 hasConcept C2777411675 @default.
- W2418515572 hasConcept C2777834122 @default.
- W2418515572 hasConcept C2778394429 @default.
- W2418515572 hasConcept C2778939058 @default.
- W2418515572 hasConcept C2779404806 @default.
- W2418515572 hasConcept C54355233 @default.
- W2418515572 hasConcept C55493867 @default.
- W2418515572 hasConcept C71924100 @default.
- W2418515572 hasConcept C81885089 @default.
- W2418515572 hasConcept C86803240 @default.
- W2418515572 hasConceptScore W2418515572C123012128 @default.
- W2418515572 hasConceptScore W2418515572C153911025 @default.
- W2418515572 hasConceptScore W2418515572C16685009 @default.
- W2418515572 hasConceptScore W2418515572C181199279 @default.
- W2418515572 hasConceptScore W2418515572C185592680 @default.
- W2418515572 hasConceptScore W2418515572C202751555 @default.
- W2418515572 hasConceptScore W2418515572C203014093 @default.
- W2418515572 hasConceptScore W2418515572C2776955114 @default.
- W2418515572 hasConceptScore W2418515572C2777411675 @default.
- W2418515572 hasConceptScore W2418515572C2777834122 @default.
- W2418515572 hasConceptScore W2418515572C2778394429 @default.
- W2418515572 hasConceptScore W2418515572C2778939058 @default.
- W2418515572 hasConceptScore W2418515572C2779404806 @default.
- W2418515572 hasConceptScore W2418515572C54355233 @default.
- W2418515572 hasConceptScore W2418515572C55493867 @default.
- W2418515572 hasConceptScore W2418515572C71924100 @default.
- W2418515572 hasConceptScore W2418515572C81885089 @default.
- W2418515572 hasConceptScore W2418515572C86803240 @default.
- W2418515572 hasIssue "2" @default.
- W2418515572 hasLocation W24185155721 @default.
- W2418515572 hasLocation W24185155722 @default.
- W2418515572 hasOpenAccess W2418515572 @default.
- W2418515572 hasPrimaryLocation W24185155721 @default.
- W2418515572 hasRelatedWork W1412312163 @default.
- W2418515572 hasRelatedWork W1494366070 @default.
- W2418515572 hasRelatedWork W2351088649 @default.
- W2418515572 hasRelatedWork W2352277170 @default.
- W2418515572 hasRelatedWork W2354517276 @default.
- W2418515572 hasRelatedWork W2364151473 @default.
- W2418515572 hasRelatedWork W2370336809 @default.
- W2418515572 hasRelatedWork W2370566711 @default.
- W2418515572 hasRelatedWork W2378429180 @default.
- W2418515572 hasRelatedWork W2381383858 @default.
- W2418515572 hasRelatedWork W2398115116 @default.
- W2418515572 hasRelatedWork W2414154260 @default.
- W2418515572 hasRelatedWork W2416795074 @default.
- W2418515572 hasRelatedWork W24205338 @default.
- W2418515572 hasRelatedWork W2460337720 @default.
- W2418515572 hasRelatedWork W2787214513 @default.
- W2418515572 hasRelatedWork W2998415739 @default.
- W2418515572 hasRelatedWork W3146369806 @default.
- W2418515572 hasRelatedWork W2572592555 @default.
- W2418515572 hasRelatedWork W3035962692 @default.
- W2418515572 hasVolume "25" @default.
- W2418515572 isParatext "false" @default.
- W2418515572 isRetracted "false" @default.
- W2418515572 magId "2418515572" @default.
- W2418515572 workType "article" @default.