Matches in SemOpenAlex for { <https://semopenalex.org/work/W2418747985> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W2418747985 endingPage "274" @default.
- W2418747985 startingPage "261" @default.
- W2418747985 abstract "The zebrafish (Danio rerio) is increasingly used as a model for studying infectious diseases. This nonmammalian vertebrate host, which is transparent at the early life stages, is especially attractive for live imaging of interactions between pathogens and host cells. A number of useful fluorescent reporter lines have recently been developed and significant advances in RNA sequencing technology have been made, which now make it possible to apply the zebrafish model for investigating changes in transcriptional activity of specific immune cell types during the course of an infection process. Here we describe how to sequence RNA extracted from fluorescently labeled macrophages obtained by cell-sorting of 5-day-old zebrafish larvae of the transgenic Tg(mpeg1:Gal4-VP16);Tg(UAS-E1b:Kaede) line. This technique showed reproducible results and allowed to detect specific expression of macrophage markers in the mpeg1 positive cell population, whereas no markers specific for neutrophils or lymphoid cells were detected. This protocol has been also successfully extended to other immune cell types as well as cells infected by Mycobacterium marinum." @default.
- W2418747985 created "2016-06-24" @default.
- W2418747985 creator A5025799952 @default.
- W2418747985 creator A5029387308 @default.
- W2418747985 creator A5063286596 @default.
- W2418747985 creator A5072386803 @default.
- W2418747985 creator A5073599090 @default.
- W2418747985 creator A5079226834 @default.
- W2418747985 date "2014-01-01" @default.
- W2418747985 modified "2023-10-04" @default.
- W2418747985 title "RNA Sequencing of FACS-Sorted Immune Cell Populations from Zebrafish Infection Models to Identify Cell Specific Responses to Intracellular Pathogens" @default.
- W2418747985 cites W1495400959 @default.
- W2418747985 cites W1989367702 @default.
- W2418747985 cites W2052297861 @default.
- W2418747985 cites W2061740109 @default.
- W2418747985 cites W2067655607 @default.
- W2418747985 cites W2073358150 @default.
- W2418747985 cites W2075271267 @default.
- W2418747985 cites W2097065948 @default.
- W2418747985 cites W2107493728 @default.
- W2418747985 cites W2114104545 @default.
- W2418747985 cites W2121690903 @default.
- W2418747985 cites W2152239989 @default.
- W2418747985 cites W2154431984 @default.
- W2418747985 doi "https://doi.org/10.1007/978-1-4939-1261-2_15" @default.
- W2418747985 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25172286" @default.
- W2418747985 hasPublicationYear "2014" @default.
- W2418747985 type Work @default.
- W2418747985 sameAs 2418747985 @default.
- W2418747985 citedByCount "27" @default.
- W2418747985 countsByYear W24187479852014 @default.
- W2418747985 countsByYear W24187479852015 @default.
- W2418747985 countsByYear W24187479852016 @default.
- W2418747985 countsByYear W24187479852017 @default.
- W2418747985 countsByYear W24187479852018 @default.
- W2418747985 countsByYear W24187479852019 @default.
- W2418747985 countsByYear W24187479852020 @default.
- W2418747985 countsByYear W24187479852021 @default.
- W2418747985 countsByYear W24187479852022 @default.
- W2418747985 countsByYear W24187479852023 @default.
- W2418747985 crossrefType "book-chapter" @default.
- W2418747985 hasAuthorship W2418747985A5025799952 @default.
- W2418747985 hasAuthorship W2418747985A5029387308 @default.
- W2418747985 hasAuthorship W2418747985A5063286596 @default.
- W2418747985 hasAuthorship W2418747985A5072386803 @default.
- W2418747985 hasAuthorship W2418747985A5073599090 @default.
- W2418747985 hasAuthorship W2418747985A5079226834 @default.
- W2418747985 hasConcept C104317684 @default.
- W2418747985 hasConcept C144024400 @default.
- W2418747985 hasConcept C1491633281 @default.
- W2418747985 hasConcept C149923435 @default.
- W2418747985 hasConcept C153726182 @default.
- W2418747985 hasConcept C189014844 @default.
- W2418747985 hasConcept C203014093 @default.
- W2418747985 hasConcept C2776878037 @default.
- W2418747985 hasConcept C2778630268 @default.
- W2418747985 hasConcept C2780384893 @default.
- W2418747985 hasConcept C2908647359 @default.
- W2418747985 hasConcept C54355233 @default.
- W2418747985 hasConcept C67705224 @default.
- W2418747985 hasConcept C86803240 @default.
- W2418747985 hasConcept C8891405 @default.
- W2418747985 hasConcept C95444343 @default.
- W2418747985 hasConceptScore W2418747985C104317684 @default.
- W2418747985 hasConceptScore W2418747985C144024400 @default.
- W2418747985 hasConceptScore W2418747985C1491633281 @default.
- W2418747985 hasConceptScore W2418747985C149923435 @default.
- W2418747985 hasConceptScore W2418747985C153726182 @default.
- W2418747985 hasConceptScore W2418747985C189014844 @default.
- W2418747985 hasConceptScore W2418747985C203014093 @default.
- W2418747985 hasConceptScore W2418747985C2776878037 @default.
- W2418747985 hasConceptScore W2418747985C2778630268 @default.
- W2418747985 hasConceptScore W2418747985C2780384893 @default.
- W2418747985 hasConceptScore W2418747985C2908647359 @default.
- W2418747985 hasConceptScore W2418747985C54355233 @default.
- W2418747985 hasConceptScore W2418747985C67705224 @default.
- W2418747985 hasConceptScore W2418747985C86803240 @default.
- W2418747985 hasConceptScore W2418747985C8891405 @default.
- W2418747985 hasConceptScore W2418747985C95444343 @default.
- W2418747985 hasLocation W24187479851 @default.
- W2418747985 hasLocation W24187479852 @default.
- W2418747985 hasOpenAccess W2418747985 @default.
- W2418747985 hasPrimaryLocation W24187479851 @default.
- W2418747985 hasRelatedWork W2105692280 @default.
- W2418747985 hasRelatedWork W2417075912 @default.
- W2418747985 hasRelatedWork W2487137350 @default.
- W2418747985 hasRelatedWork W2950711588 @default.
- W2418747985 hasRelatedWork W2978136769 @default.
- W2418747985 hasRelatedWork W3119879095 @default.
- W2418747985 hasRelatedWork W3123575957 @default.
- W2418747985 hasRelatedWork W3157458363 @default.
- W2418747985 hasRelatedWork W3208363803 @default.
- W2418747985 hasRelatedWork W4206997428 @default.
- W2418747985 isParatext "false" @default.
- W2418747985 isRetracted "false" @default.
- W2418747985 magId "2418747985" @default.
- W2418747985 workType "book-chapter" @default.