Matches in SemOpenAlex for { <https://semopenalex.org/work/W2418843084> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W2418843084 endingPage "8" @default.
- W2418843084 startingPage "704" @default.
- W2418843084 abstract "To evaluate the sensitivity and specificity of immunohistochemical (IHC) staining of DNA mismatch repair (MMR) protein for the screening of microsatellite instability (MSI) colorectal cancer (CRC).A total of 255 CRC cases were studied, including 140 cases of routine paraffin-embedded tissue samples and 115 cases constructed on tissue microarray. Expressions of 4 MMR proteins including MHL1, MSH2, MSH6 and PMS2 were investigated by IHC. Negative protein expression was defined as complete absence of nuclear staining within tumor cells in the presence of positively labeled internal non-neoplastic cells. Focal staining was defined as the presence of staining in < 5% of the tumor cells. CRCs showing negative staining for any MMR proteins were interpreted as MMR deficient tumors. PCR-genescan MSI analysis was performed in each case by a five marker panel including Bat26, Bat25, NR-21, NR-24 and MONO-27.Among the 140 CRCs with routine formalin-fixed paraffin embedded tissue sections, concordance rate between IHC and PCR-genescan was 98.6% (138/140), the sensitivity and specificity of IHC in detecting MSI tumors were 94.9% (37/39) and 100.0% (101/101), respectively. The 2 disconcordant cases showed focal staining in at least one of the MMR proteins but were confirmed to be MSI-H CRCs by PCR-genescan assay. On tissue microarray, 91.3% (105/115) of the cases had informative results. The concordance rate between IHC and PCR-genescan was 100.0% (105/105). Both the specificity and sensitivity of IHC in detecting MSI tumors on available tissue microarray samples were 100.0%. Ten cases were inclusive due to the presence of negative stains of MMR proteins in both the tumor and internal control cells.Detection of 4 MMR proteins expression by IHC is reliable for identifying MSI CRCs and is recommended for routine practice. Tumors with focal MMR protein staining are highly suspected for the presence of MSI-H and PCR-genescan based MSI analysis should be performed to confirm." @default.
- W2418843084 created "2016-06-24" @default.
- W2418843084 creator A5015302215 @default.
- W2418843084 creator A5017716773 @default.
- W2418843084 creator A5019961590 @default.
- W2418843084 creator A5032964728 @default.
- W2418843084 creator A5044777574 @default.
- W2418843084 creator A5046603959 @default.
- W2418843084 creator A5059755665 @default.
- W2418843084 creator A5081148554 @default.
- W2418843084 date "2015-10-01" @default.
- W2418843084 modified "2023-09-27" @default.
- W2418843084 title "[Value of detection of DNA mismatch repair proteins deficiency by immunohistochemistry in predicting tumor microsatellite status]." @default.
- W2418843084 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26702526" @default.
- W2418843084 hasPublicationYear "2015" @default.
- W2418843084 type Work @default.
- W2418843084 sameAs 2418843084 @default.
- W2418843084 citedByCount "4" @default.
- W2418843084 countsByYear W24188430842020 @default.
- W2418843084 countsByYear W24188430842021 @default.
- W2418843084 countsByYear W24188430842023 @default.
- W2418843084 crossrefType "journal-article" @default.
- W2418843084 hasAuthorship W2418843084A5015302215 @default.
- W2418843084 hasAuthorship W2418843084A5017716773 @default.
- W2418843084 hasAuthorship W2418843084A5019961590 @default.
- W2418843084 hasAuthorship W2418843084A5032964728 @default.
- W2418843084 hasAuthorship W2418843084A5044777574 @default.
- W2418843084 hasAuthorship W2418843084A5046603959 @default.
- W2418843084 hasAuthorship W2418843084A5059755665 @default.
- W2418843084 hasAuthorship W2418843084A5081148554 @default.
- W2418843084 hasConcept C104317684 @default.
- W2418843084 hasConcept C121608353 @default.
- W2418843084 hasConcept C142724271 @default.
- W2418843084 hasConcept C153911025 @default.
- W2418843084 hasConcept C160798450 @default.
- W2418843084 hasConcept C180754005 @default.
- W2418843084 hasConcept C193270364 @default.
- W2418843084 hasConcept C204232928 @default.
- W2418843084 hasConcept C2776559941 @default.
- W2418843084 hasConcept C2776674815 @default.
- W2418843084 hasConcept C2778677574 @default.
- W2418843084 hasConcept C2779115348 @default.
- W2418843084 hasConcept C2779767149 @default.
- W2418843084 hasConcept C526805850 @default.
- W2418843084 hasConcept C54355233 @default.
- W2418843084 hasConcept C60644358 @default.
- W2418843084 hasConcept C60748783 @default.
- W2418843084 hasConcept C61320498 @default.
- W2418843084 hasConcept C71924100 @default.
- W2418843084 hasConcept C74864618 @default.
- W2418843084 hasConcept C86803240 @default.
- W2418843084 hasConceptScore W2418843084C104317684 @default.
- W2418843084 hasConceptScore W2418843084C121608353 @default.
- W2418843084 hasConceptScore W2418843084C142724271 @default.
- W2418843084 hasConceptScore W2418843084C153911025 @default.
- W2418843084 hasConceptScore W2418843084C160798450 @default.
- W2418843084 hasConceptScore W2418843084C180754005 @default.
- W2418843084 hasConceptScore W2418843084C193270364 @default.
- W2418843084 hasConceptScore W2418843084C204232928 @default.
- W2418843084 hasConceptScore W2418843084C2776559941 @default.
- W2418843084 hasConceptScore W2418843084C2776674815 @default.
- W2418843084 hasConceptScore W2418843084C2778677574 @default.
- W2418843084 hasConceptScore W2418843084C2779115348 @default.
- W2418843084 hasConceptScore W2418843084C2779767149 @default.
- W2418843084 hasConceptScore W2418843084C526805850 @default.
- W2418843084 hasConceptScore W2418843084C54355233 @default.
- W2418843084 hasConceptScore W2418843084C60644358 @default.
- W2418843084 hasConceptScore W2418843084C60748783 @default.
- W2418843084 hasConceptScore W2418843084C61320498 @default.
- W2418843084 hasConceptScore W2418843084C71924100 @default.
- W2418843084 hasConceptScore W2418843084C74864618 @default.
- W2418843084 hasConceptScore W2418843084C86803240 @default.
- W2418843084 hasIssue "10" @default.
- W2418843084 hasLocation W24188430841 @default.
- W2418843084 hasOpenAccess W2418843084 @default.
- W2418843084 hasPrimaryLocation W24188430841 @default.
- W2418843084 hasRelatedWork W1990603731 @default.
- W2418843084 hasRelatedWork W1995795701 @default.
- W2418843084 hasRelatedWork W2056707782 @default.
- W2418843084 hasRelatedWork W2125477979 @default.
- W2418843084 hasRelatedWork W2318611309 @default.
- W2418843084 hasRelatedWork W2328839624 @default.
- W2418843084 hasRelatedWork W2418843084 @default.
- W2418843084 hasRelatedWork W3010691660 @default.
- W2418843084 hasRelatedWork W3040417528 @default.
- W2418843084 hasRelatedWork W3141874892 @default.
- W2418843084 hasVolume "44" @default.
- W2418843084 isParatext "false" @default.
- W2418843084 isRetracted "false" @default.
- W2418843084 magId "2418843084" @default.
- W2418843084 workType "article" @default.