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- W2419008053 abstract "Perlecan, the major heparan sulfate (HS) proteoglycan of basement membranes and other connective tissues, is a modular molecule with five structural domains. It has been isolated from various sources including kidney and placenta, but most commonly from the mouse Englebroth Holm Swarm (EHS) tumor (1,2). It has also been purified from cultured cell lines such as endothelial cells (3) or fibroblasts (4). When it has been extracted from tissue or from the extracellular matrix of cultured cells, it has been first solubilized using chaotropic agents such as urea or guanidine. The effect of these dissociating agents on the structure and function of isolated proteoglycans is not known, but it is thought that some of the denaturation may be irreversible. Human perlecan is found predominantly as a heparan sulfate proteoglycan, but has been isolated from carcinoma cell lines as an undecorated protein core and from placental tissue decorated with chondroitin sulfate/dermatan sulfate. The molecular weight of the protein core is approximately 470 kDa, while the size of the mouse homolog is around 400 kDa. Domain I is the N-terminal domain, and it contains a cluster of three potential glycosaminoglycan attachment sites. Domain II has homology to the LDL receptor, with strict conservation of the cysteine residue positions. Domain III shares homology with the short arm of the laminin α-chain. Domain IV is the largest domain with a molecular weight in excess of 200 kDa and is made up of numerous immunoglobulin-like repeats similar to those found in the neural cell adhesion molecule. Domain V is the C-terminal domain, and is made up of three regions with homology to the globular domains of the laminin α-chain, interspersed with four epidermal growth factor-like repeats." @default.
- W2419008053 created "2016-06-24" @default.
- W2419008053 creator A5024041758 @default.
- W2419008053 date "2003-11-14" @default.
- W2419008053 modified "2023-10-18" @default.
- W2419008053 title "Purification of Perlecan from Endothelial Cells" @default.
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- W2419008053 doi "https://doi.org/10.1385/1-59259-209-0:027" @default.
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