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- W2419012247 abstract "A new enzymatic fluorimetric assay for the determination of total serum bile acids is described. The enzyme 3 alpha-idroxysteroid NAD oxireductase by oxydising the free OH in position 3 alpha of bile acid molecule, reduce NAD previously added. Afterwards the hydrogen of the generated NADH is transferred by a diaphorase to resazurin to yield the fluorophore resorufin proportionally to the amount of bile acids in the sample. The assay is simple, accurate, without bilirubin's influence and useful for a routine use because the analysis time is not different from the more common laboratory tests." @default.
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- W2419012247 date "1980-02-25" @default.
- W2419012247 modified "2023-09-27" @default.
- W2419012247 title "[Spectrophotofluorimetric enzymatic determination of total serum bile acids]." @default.
- W2419012247 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6928510" @default.
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