Matches in SemOpenAlex for { <https://semopenalex.org/work/W2419015454> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W2419015454 abstract "A novel endodextranase isolated from Paenibacillus sp. was found to produce isomaltotetraose and small amounts of cycloisomaltooligosaccharides with a degree of polymerization of 7-14 from dextran. To determine the active site, the enzyme was modified with 1-ethyl-3-[3- (dimethylamino)-propyl]-carbodiimide (EDC) and α-epoxyalkyl α-glucosides (EAGs), an affinity labeling reagent. The inactivation followed pseudo first-order kinetics. Kinetic analysis and chemical modification using EDC and EAGs indicated that carboxyl groups are essential for the enzymatic activity. Three Asp and one Glu residues were identified as candidate catalytic amino acids, since these residues are completely conserved across the GH family of 66 enzymes. Replacement of Asp189, Asp340, or Glu412 completely abolished the enzyme activity, indicating that these residues are essential for catalytic activity." @default.
- W2419015454 created "2016-06-24" @default.
- W2419015454 creator A5007961488 @default.
- W2419015454 creator A5012770069 @default.
- W2419015454 creator A5015629051 @default.
- W2419015454 creator A5030467675 @default.
- W2419015454 creator A5060167939 @default.
- W2419015454 date "2016-05-28" @default.
- W2419015454 modified "2023-10-16" @default.
- W2419015454 title "Identification of Catalytic Amino Acid Residues by Chemical Modification in Dextranase" @default.
- W2419015454 cites W1497245978 @default.
- W2419015454 cites W1527522123 @default.
- W2419015454 cites W1553947817 @default.
- W2419015454 cites W1557998611 @default.
- W2419015454 cites W1562322062 @default.
- W2419015454 cites W1826776293 @default.
- W2419015454 cites W1830745998 @default.
- W2419015454 cites W1985546596 @default.
- W2419015454 cites W1986248763 @default.
- W2419015454 cites W1987336087 @default.
- W2419015454 cites W2026372724 @default.
- W2419015454 cites W2050121959 @default.
- W2419015454 cites W2061241518 @default.
- W2419015454 cites W2062815986 @default.
- W2419015454 cites W2064162477 @default.
- W2419015454 cites W2074480464 @default.
- W2419015454 cites W2079179890 @default.
- W2419015454 cites W2087634551 @default.
- W2419015454 cites W2088754568 @default.
- W2419015454 cites W2128635872 @default.
- W2419015454 cites W2137843727 @default.
- W2419015454 cites W2151413823 @default.
- W2419015454 cites W2168407253 @default.
- W2419015454 cites W2396372938 @default.
- W2419015454 cites W2509589851 @default.
- W2419015454 cites W68909338 @default.
- W2419015454 doi "https://doi.org/10.4014/jmb.1601.01014" @default.
- W2419015454 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26907761" @default.
- W2419015454 hasPublicationYear "2016" @default.
- W2419015454 type Work @default.
- W2419015454 sameAs 2419015454 @default.
- W2419015454 citedByCount "2" @default.
- W2419015454 countsByYear W24190154542016 @default.
- W2419015454 countsByYear W24190154542019 @default.
- W2419015454 crossrefType "journal-article" @default.
- W2419015454 hasAuthorship W2419015454A5007961488 @default.
- W2419015454 hasAuthorship W2419015454A5012770069 @default.
- W2419015454 hasAuthorship W2419015454A5015629051 @default.
- W2419015454 hasAuthorship W2419015454A5030467675 @default.
- W2419015454 hasAuthorship W2419015454A5060167939 @default.
- W2419015454 hasConcept C161790260 @default.
- W2419015454 hasConcept C178790620 @default.
- W2419015454 hasConcept C181199279 @default.
- W2419015454 hasConcept C184133267 @default.
- W2419015454 hasConcept C185592680 @default.
- W2419015454 hasConcept C2775997276 @default.
- W2419015454 hasConcept C2776183398 @default.
- W2419015454 hasConcept C41183919 @default.
- W2419015454 hasConcept C515207424 @default.
- W2419015454 hasConcept C55493867 @default.
- W2419015454 hasConcept C71240020 @default.
- W2419015454 hasConceptScore W2419015454C161790260 @default.
- W2419015454 hasConceptScore W2419015454C178790620 @default.
- W2419015454 hasConceptScore W2419015454C181199279 @default.
- W2419015454 hasConceptScore W2419015454C184133267 @default.
- W2419015454 hasConceptScore W2419015454C185592680 @default.
- W2419015454 hasConceptScore W2419015454C2775997276 @default.
- W2419015454 hasConceptScore W2419015454C2776183398 @default.
- W2419015454 hasConceptScore W2419015454C41183919 @default.
- W2419015454 hasConceptScore W2419015454C515207424 @default.
- W2419015454 hasConceptScore W2419015454C55493867 @default.
- W2419015454 hasConceptScore W2419015454C71240020 @default.
- W2419015454 hasLocation W24190154541 @default.
- W2419015454 hasLocation W24190154542 @default.
- W2419015454 hasOpenAccess W2419015454 @default.
- W2419015454 hasPrimaryLocation W24190154541 @default.
- W2419015454 hasRelatedWork W1550634984 @default.
- W2419015454 hasRelatedWork W2004312381 @default.
- W2419015454 hasRelatedWork W2017880409 @default.
- W2419015454 hasRelatedWork W2043316009 @default.
- W2419015454 hasRelatedWork W2051302445 @default.
- W2419015454 hasRelatedWork W2170946482 @default.
- W2419015454 hasRelatedWork W2409612133 @default.
- W2419015454 hasRelatedWork W2419015454 @default.
- W2419015454 hasRelatedWork W2900504840 @default.
- W2419015454 hasRelatedWork W3131068657 @default.
- W2419015454 isParatext "false" @default.
- W2419015454 isRetracted "false" @default.
- W2419015454 magId "2419015454" @default.
- W2419015454 workType "article" @default.