FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2419098047> ?p ?o ?g. }

• W2419098047 endingPage "362" @default.
• W2419098047 startingPage "351" @default.
• W2419098047 abstract "A large-scale approach to the purification of plasmid DNA has been developed that overcomes many of the limitations of current chromatography-based processes. The process consists of a scaleable lysis using recombinant lysozyme and a rapid heating and cooling step followed by a selective precipitation with cetyltrimethylammonium bromide (CTAB). Calcium silicate batch adsorption is then utilized to remove residual genomic DNA, linear plasmid, open circular plasmid, endotoxin, detergents, and proteins. Finally, a concentration and diafiltration step utilizing ultrafiltration and a terminal sterile filtration complete the process. The final product exceeds the requirements for clinical-grade plasmid DNA, and the process has been scaled up to yield an average of 18 ± 4 g (over five lots) of pharmaceutically pure plasmid DNA per 140 L of lysate (from approx 1.3 kg Escherichia coli dry cell weight)." @default.
• W2419098047 created "2016-06-24" @default.
• W2419098047 creator A5016944934 @default.
• W2419098047 creator A5074117844 @default.
• W2419098047 creator A5074458832 @default.
• W2419098047 date "2006-09-20" @default.
• W2419098047 modified "2023-09-24" @default.
• W2419098047 title "Large-Scale, Nonchromatographic Purification of Plasmid DNA" @default.
• W2419098047 cites W1502866179 @default.
• W2419098047 cites W1963516326 @default.
• W2419098047 cites W1984471128 @default.
• W2419098047 cites W2005543617 @default.
• W2419098047 cites W2007146866 @default.
• W2419098047 cites W2014932633 @default.
• W2419098047 cites W2015242294 @default.
• W2419098047 cites W2017290048 @default.
• W2419098047 cites W2019410656 @default.
• W2419098047 cites W2061938613 @default.
• W2419098047 cites W2092786524 @default.
• W2419098047 cites W2125516773 @default.
• W2419098047 cites W2138476456 @default.
• W2419098047 cites W2167949866 @default.
• W2419098047 cites W2419380752 @default.
• W2419098047 cites W2793856065 @default.
• W2419098047 cites W2158061278 @default.
• W2419098047 cites W2411640546 @default.
• W2419098047 doi "https://doi.org/10.1385/1-59745-168-1:351" @default.
• W2419098047 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16988465" @default.
• W2419098047 hasPublicationYear "2006" @default.
• W2419098047 type Work @default.
• W2419098047 sameAs 2419098047 @default.
• W2419098047 citedByCount "7" @default.
• W2419098047 countsByYear W24190980472014 @default.
• W2419098047 countsByYear W24190980472015 @default.
• W2419098047 countsByYear W24190980472016 @default.
• W2419098047 countsByYear W24190980472021 @default.
• W2419098047 crossrefType "book-chapter" @default.
• W2419098047 hasAuthorship W2419098047A5016944934 @default.
• W2419098047 hasAuthorship W2419098047A5074117844 @default.
• W2419098047 hasAuthorship W2419098047A5074458832 @default.
• W2419098047 hasConcept C104317684 @default.
• W2419098047 hasConcept C105795698 @default.
• W2419098047 hasConcept C109932267 @default.
• W2419098047 hasConcept C128489963 @default.
• W2419098047 hasConcept C134121241 @default.
• W2419098047 hasConcept C150394285 @default.
• W2419098047 hasConcept C167508917 @default.
• W2419098047 hasConcept C178790620 @default.
• W2419098047 hasConcept C181199279 @default.
• W2419098047 hasConcept C185592680 @default.
• W2419098047 hasConcept C18743360 @default.
• W2419098047 hasConcept C191897082 @default.
• W2419098047 hasConcept C192562407 @default.
• W2419098047 hasConcept C193363816 @default.
• W2419098047 hasConcept C205225487 @default.
• W2419098047 hasConcept C20808630 @default.
• W2419098047 hasConcept C22744801 @default.
• W2419098047 hasConcept C33923547 @default.
• W2419098047 hasConcept C40767141 @default.
• W2419098047 hasConcept C41625074 @default.
• W2419098047 hasConcept C43617362 @default.
• W2419098047 hasConcept C48314217 @default.
• W2419098047 hasConcept C50817676 @default.
• W2419098047 hasConcept C53163501 @default.
• W2419098047 hasConcept C547475151 @default.
• W2419098047 hasConcept C552990157 @default.
• W2419098047 hasConcept C55493867 @default.
• W2419098047 hasConcept C57409179 @default.
• W2419098047 hasConceptScore W2419098047C104317684 @default.
• W2419098047 hasConceptScore W2419098047C105795698 @default.
• W2419098047 hasConceptScore W2419098047C109932267 @default.
• W2419098047 hasConceptScore W2419098047C128489963 @default.
• W2419098047 hasConceptScore W2419098047C134121241 @default.
• W2419098047 hasConceptScore W2419098047C150394285 @default.
• W2419098047 hasConceptScore W2419098047C167508917 @default.
• W2419098047 hasConceptScore W2419098047C178790620 @default.
• W2419098047 hasConceptScore W2419098047C181199279 @default.
• W2419098047 hasConceptScore W2419098047C185592680 @default.
• W2419098047 hasConceptScore W2419098047C18743360 @default.
• W2419098047 hasConceptScore W2419098047C191897082 @default.
• W2419098047 hasConceptScore W2419098047C192562407 @default.
• W2419098047 hasConceptScore W2419098047C193363816 @default.
• W2419098047 hasConceptScore W2419098047C205225487 @default.
• W2419098047 hasConceptScore W2419098047C20808630 @default.
• W2419098047 hasConceptScore W2419098047C22744801 @default.
• W2419098047 hasConceptScore W2419098047C33923547 @default.
• W2419098047 hasConceptScore W2419098047C40767141 @default.
• W2419098047 hasConceptScore W2419098047C41625074 @default.
• W2419098047 hasConceptScore W2419098047C43617362 @default.
• W2419098047 hasConceptScore W2419098047C48314217 @default.
• W2419098047 hasConceptScore W2419098047C50817676 @default.
• W2419098047 hasConceptScore W2419098047C53163501 @default.
• W2419098047 hasConceptScore W2419098047C547475151 @default.
• W2419098047 hasConceptScore W2419098047C552990157 @default.
• W2419098047 hasConceptScore W2419098047C55493867 @default.
• W2419098047 hasConceptScore W2419098047C57409179 @default.
• W2419098047 hasLocation W24190980471 @default.
• W2419098047 hasLocation W24190980472 @default.

• next