FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2419149082> ?p ?o ?g. }

• W2419149082 endingPage "2474" @default.
• W2419149082 startingPage "2474" @default.
• W2419149082 abstract "To establish an efficient, sensitive, cell-based assay system for NS3 serine protease in an effort to study further the property of hepatitis C virus (HCV) and develop new antiviral agents.We constructed pCI-neo-NS3/4A-SEAP chimeric plasmid, in which the secreted alkaline phosphatase (SEAP) was fused in-frame to the downstream of NS4A/4B cleavage site. The protease activity of NS3 was reflected by the activity of SEAP in the culture media of transient or stable expression cells. Stably expressing cell lines were obtained by G418 selection. Pefabloc SC, a potent irreversible serine protease inhibitor, was used to treat the stably expressing cell lines to assess the system for screening NS3 inhibitors. To compare the activity of serine proteases from 1b and 1a, two chimeric clones were constructed and introduced into both transient and stable expression systems.The SEAP activity in the culture media could be detected in both transient and stable expression systems, and was apparently decreased after Pefabloc SC treatment. In both transient and stable systems, NS3/4A-SEAP chimeric gene from HCV genotype 1b produced higher SEAP activity in the culture media than that from 1a.The cell-based system is efficient and sensitive enough for detection and comparison of NS3 protease activity, and screening of anti-NS3 inhibitors. The functional difference between NS3/4A from 1a and 1b subtypes revealed by this system provides a clue for further investigations." @default.
• W2419149082 created "2016-06-24" @default.
• W2419149082 creator A5002265583 @default.
• W2419149082 creator A5021524118 @default.
• W2419149082 creator A5042519138 @default.
• W2419149082 creator A5071233167 @default.
• W2419149082 creator A5090384031 @default.
• W2419149082 date "2003-01-01" @default.
• W2419149082 modified "2023-09-25" @default.
• W2419149082 title "Establishment of a cell-based assay system for hepatitis C virus serine protease and its primary applications" @default.
• W2419149082 cites W145836047 @default.
• W2419149082 cites W1506258653 @default.
• W2419149082 cites W1542447311 @default.
• W2419149082 cites W1574175588 @default.
• W2419149082 cites W1820552472 @default.
• W2419149082 cites W1973165481 @default.
• W2419149082 cites W1974238871 @default.
• W2419149082 cites W1982957818 @default.
• W2419149082 cites W1984396607 @default.
• W2419149082 cites W1985861913 @default.
• W2419149082 cites W2004660555 @default.
• W2419149082 cites W2012797613 @default.
• W2419149082 cites W2017101209 @default.
• W2419149082 cites W2028200392 @default.
• W2419149082 cites W2059460158 @default.
• W2419149082 cites W2060162386 @default.
• W2419149082 cites W2069726305 @default.
• W2419149082 cites W2071952696 @default.
• W2419149082 cites W2078887917 @default.
• W2419149082 cites W2081941928 @default.
• W2419149082 cites W2084324934 @default.
• W2419149082 cites W2085344362 @default.
• W2419149082 cites W2117224415 @default.
• W2419149082 cites W2122742989 @default.
• W2419149082 cites W2124000798 @default.
• W2419149082 cites W2126153046 @default.
• W2419149082 cites W2140206005 @default.
• W2419149082 cites W2149270925 @default.
• W2419149082 cites W2151898297 @default.
• W2419149082 cites W2158021717 @default.
• W2419149082 cites W2161945006 @default.
• W2419149082 cites W2188870463 @default.
• W2419149082 cites W2403438648 @default.
• W2419149082 doi "https://doi.org/10.3748/wjg.v9.i11.2474" @default.
• W2419149082 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4656523" @default.
• W2419149082 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14606079" @default.
• W2419149082 hasPublicationYear "2003" @default.
• W2419149082 type Work @default.
• W2419149082 sameAs 2419149082 @default.
• W2419149082 citedByCount "10" @default.
• W2419149082 countsByYear W24191490822013 @default.
• W2419149082 countsByYear W24191490822023 @default.
• W2419149082 crossrefType "journal-article" @default.
• W2419149082 hasAuthorship W2419149082A5002265583 @default.
• W2419149082 hasAuthorship W2419149082A5021524118 @default.
• W2419149082 hasAuthorship W2419149082A5042519138 @default.
• W2419149082 hasAuthorship W2419149082A5071233167 @default.
• W2419149082 hasAuthorship W2419149082A5090384031 @default.
• W2419149082 hasBestOaLocation W24191490821 @default.
• W2419149082 hasConcept C132379496 @default.
• W2419149082 hasConcept C153911025 @default.
• W2419149082 hasConcept C159047783 @default.
• W2419149082 hasConcept C181199279 @default.
• W2419149082 hasConcept C182220744 @default.
• W2419149082 hasConcept C185592680 @default.
• W2419149082 hasConcept C2522874641 @default.
• W2419149082 hasConcept C2776408679 @default.
• W2419149082 hasConcept C2776414213 @default.
• W2419149082 hasConcept C2776714187 @default.
• W2419149082 hasConcept C2777807008 @default.
• W2419149082 hasConcept C54355233 @default.
• W2419149082 hasConcept C55493867 @default.
• W2419149082 hasConcept C81885089 @default.
• W2419149082 hasConcept C82553315 @default.
• W2419149082 hasConcept C83100098 @default.
• W2419149082 hasConcept C86803240 @default.
• W2419149082 hasConceptScore W2419149082C132379496 @default.
• W2419149082 hasConceptScore W2419149082C153911025 @default.
• W2419149082 hasConceptScore W2419149082C159047783 @default.
• W2419149082 hasConceptScore W2419149082C181199279 @default.
• W2419149082 hasConceptScore W2419149082C182220744 @default.
• W2419149082 hasConceptScore W2419149082C185592680 @default.
• W2419149082 hasConceptScore W2419149082C2522874641 @default.
• W2419149082 hasConceptScore W2419149082C2776408679 @default.
• W2419149082 hasConceptScore W2419149082C2776414213 @default.
• W2419149082 hasConceptScore W2419149082C2776714187 @default.
• W2419149082 hasConceptScore W2419149082C2777807008 @default.
• W2419149082 hasConceptScore W2419149082C54355233 @default.
• W2419149082 hasConceptScore W2419149082C55493867 @default.
• W2419149082 hasConceptScore W2419149082C81885089 @default.
• W2419149082 hasConceptScore W2419149082C82553315 @default.
• W2419149082 hasConceptScore W2419149082C83100098 @default.
• W2419149082 hasConceptScore W2419149082C86803240 @default.
• W2419149082 hasIssue "11" @default.
• W2419149082 hasLocation W24191490821 @default.
• W2419149082 hasLocation W24191490822 @default.
• W2419149082 hasLocation W24191490823 @default.
• W2419149082 hasLocation W24191490824 @default.

• next