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- W2419200963 abstract "Yeast YIp-type expression recombinant plasmid(pCMA2-1) was constructed. The expression of alpha-acetolactate decarboxylase gene from Bacillus subtilis was controled by CUP1 promoter and its own terminator. The recombinant plasmid pCMA2-1 was introduced into the brewer's yeast PJ3-5. Transformants were selected using copper resistance as selected marker. The results of activity assay showed that PJ3-5 didn't produce alpha-acetolactate decarboxylase(ALDC), where as the activity of alpha-ALDC in transformants were induced by copper sulfate. The laboratory scale fermentation test confirmed that the total diacetyl concentration was reduced effectively by alpha-ALDC in transformant." @default.
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- W2419200963 date "2001-02-01" @default.
- W2419200963 modified "2023-09-23" @default.
- W2419200963 title "[Expression of alpha-acetolactate decarboxylase gene from Bacillus subtilis in Brewer's yeast]." @default.
- W2419200963 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12549198" @default.
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