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• W2419204811 abstract "AbstractMany bacteria can be detected in CF sputum, pathogenic and commensal. Modified Koch’s criteria for identification of established and emerging CF pathogens are therefore described. Methods are described to isolate bacteria and to detect bacterial biofilms in sputum or lung tissue from CF patients by means of conventional culturing and staining techniques and by the PNA FISH technique. Additionally, the confocal scanning laser microscopy technique is described for studying biofilms in vitro in a flow cell system. The recA-gene PCR and the RFLP-based identification methods are described for identification of isolates from the Burkholderia complex to the species level. DNA typing by PFGE, which can be used for any bacterial pathogen, is described as it is employed for Pseudomonas aeruginosa. A commercially available ELISA method is described for measuring IgG antibodies against P. aeruginosa in CF patients.Key wordsBiofilmKoch’s criteria Pseudomonas aeruginosa Burkholderia FISH Pseudomonas antibodiespulsed field gel electrophoresisconfocal scanning laser microscopy recA-gene PCRmicrobiology" @default.
• W2419204811 created "2016-06-24" @default.
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• W2419204811 date "2011-01-01" @default.
• W2419204811 modified "2023-09-24" @default.
• W2419204811 title "Methods to Classify Bacterial Pathogens in Cystic Fibrosis" @default.
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• W2419204811 doi "https://doi.org/10.1007/978-1-61779-120-8_9" @default.
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