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- W2419247263 abstract "Previously cloned and sequenced full-length cDNAs for murine C4 and the closely related sex-limited protein (Sip) have been placed into an eucaryotic expression vector. Transfer of these DNA constructs transiently into monkey COS cells or stably into mouse L cells results in the expression and secretion of hemolytically active mouse C4 and mature Sip. We estimate from hemolytic activities that COS and L cells secrete 0.04 and 3%, respectively, of the C4 level found in mouse plasma. Sip expression is consistently only 10-20% that of C4 although the identical expression system is used for both. Our results show subtle but reproducible cell-type-specific differences in C4 maturation; they also indicate that surprisingly large shifts in electrophoretic mobility on SDS-polyacrylamide gels are induced by a small number of amino acid substitutions. The expression of C4 from cDNAs of known sequence provides a starting point for studies of structure/function relationships in C4 employing site-specific mutagenesis and gene transfer." @default.
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- W2419247263 date "1989-01-01" @default.
- W2419247263 modified "2023-10-18" @default.
- W2419247263 title "Transfected cDNA Directs Expression of Hemolytically Active Murine C4 in Cultured Mouse and Monkey Cells" @default.
- W2419247263 doi "https://doi.org/10.1159/000463113" @default.
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