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- W2419449679 abstract "As the best-known Post Translational Modifications (PTMs), protein phosphorylation has reached thestage where its importance in almost every life event is recognized. However, our understanding ofprotein phosphorylation is still in its infancy. The greatest challenge of phosphorylation analysis is due toits highly dynamic nature and low abundance. Therefore it is crucial to develop new analytical methodsfor phosphorylation analysis to separate the phosphorylated peptides from the complex samples. Hereina new reversed-phase-reversed-phase liquid chromatography (RP-RPLC) approach was developed formultidimensional separation of phosphopeptides. Combination of this multidimensional system,immobilized metal affinity chromatography, high-accuracy mass spectrometry instrumentation, and a newdata processing platform called Armone, a total of 9,995 phosphorylation sites and 3149 phosphoproteinsfrom 40mg tryptic digestion of human liver lysate were identified with an FDR 1.0%. To our knowledge,this is the largest data set for human liver phosphoproteome. This pH-based RP-RPLC technology allowsa higher coverage of phosphoproteome and holds a promising in large-scale analysis of proteinphosphorylation. Keywords: phosphorylation analysis / multidimensional chromatography /phosphopeptide / human liver / pH-reversed phase / phosphoproteomics. References: 1. Cohen, P. NatCell Biol.2002, 4, E127-E130. 2. McLachlin, D. T.; Chait, B. T. Curr Opin Chem Biol.2001, 5, 591-602. 3.Paradela, A; Albar, J. P,. J. Proteome Res. 2008, 7, 1809-1818. 4. Gilar, M; Olicoca, P; Daly, A. E;Gebler, J. C. Anal. Chem, 2005, 77, 6426-6434. 5. Villen, J; Beausoleil, S.A; Gerber, S.A; Gygi, S.P.Proc. Natl. Acad. Sci. USA, 2007, 104, 1488-1493." @default.
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- W2419449679 date "2010-08-29" @default.
- W2419449679 modified "2023-09-24" @default.
- W2419449679 title "A New RP-RPLC Approach for Large-scale Phosphorylation Analysis of Human Liver." @default.
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