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- W2419482027 abstract "<ns4:p>Here we describe the development of a protocol to make small oligomers, dimers and trimers, from highly oligomeric membrane proteins. The proteins that we used are the light harvesting 2 proteins and core complexes from photosynthetic bacteria, which contain respectively 16 and 56 individual polypeptides. Creating specific dimers between such multimeric protein poses several problems. We propose a protocol based on asymmetric lysine localization, thanks to the positive inside rule, and copper-free click chemistry. With this method we are able to produce specific dimeric complexes in detergent solution of possible biological relevance.</ns4:p>" @default.
- W2419482027 created "2016-06-24" @default.
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- W2419482027 date "2016-06-01" @default.
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- W2419482027 title "Making dimers of oligomeric membrane proteins using copper-free click chemistry" @default.
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- W2419482027 doi "https://doi.org/10.12688/f1000research.8676.1" @default.
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