Matches in SemOpenAlex for { <https://semopenalex.org/work/W2419491452> ?p ?o ?g. }
- W2419491452 endingPage "2949" @default.
- W2419491452 startingPage "2941" @default.
- W2419491452 abstract "A sensitive and validated liquid chromatography with mass spectrometry method was developed for the enantioseparation of the racemic mixture of pomalidomide, a novel, second-generation immunomodulatory drug, using β-cyclodextrin-bonded stationary phases. Four cyclodextrin columns (β-, hydroxypropyl-β-, carboxymethyl-β-, and sulfobutyl-β-cyclodextrin) were screened and the effects of eluent composition, flow rate, temperature, and organic modifier on enantioseparation were studied. Optimized parameters, offering baseline separation (resolution = 2.70 ± 0.02) were the following: β-cyclodextrin stationary phase, thermostatted at 15°C, and mobile phase consisting of methanol/0.1% acetic acid 10:90 v/v, delivered with 0.8 mL/min flow rate. For the optimized parameter at multiple reaction monitoring mode 274.1-201.0 transition with 20 eV collision energy and 100 V fragmentor voltage the limit of detection and limit of quantitation were 0.75 and 2.00 ng/mL, respectively. Since enantiopure standards were not available, elution order was determined upon comparison of the circular dichroism signals of the separated pomalidomide enantiomers with that of enantiopure thalidomide. The mechanisms underlying the chiral discrimination between the enantiomers were also investigated. Pomalidomide-β-cyclodextrin inclusion complex was characterized using nuclear magnetic resonance spectroscopy and molecular modeling. The thermodynamic aspects of chiral separation were also studied." @default.
- W2419491452 created "2016-06-24" @default.
- W2419491452 creator A5023572041 @default.
- W2419491452 creator A5031371599 @default.
- W2419491452 creator A5034145752 @default.
- W2419491452 creator A5046831186 @default.
- W2419491452 creator A5051465571 @default.
- W2419491452 creator A5062803279 @default.
- W2419491452 creator A5063493127 @default.
- W2419491452 creator A5080517520 @default.
- W2419491452 creator A5083083294 @default.
- W2419491452 date "2016-07-06" @default.
- W2419491452 modified "2023-10-17" @default.
- W2419491452 title "Liquid chromatography with mass spectrometry enantioseparation of pomalidomide on cyclodextrin-bonded chiral stationary phases and the elucidation of the chiral recognition mechanisms by NMR spectroscopy and molecular modeling" @default.
- W2419491452 cites W1967930461 @default.
- W2419491452 cites W1972113760 @default.
- W2419491452 cites W1972733339 @default.
- W2419491452 cites W1978425388 @default.
- W2419491452 cites W1981368803 @default.
- W2419491452 cites W1998154803 @default.
- W2419491452 cites W2000377437 @default.
- W2419491452 cites W2012148056 @default.
- W2419491452 cites W2016303039 @default.
- W2419491452 cites W2021315742 @default.
- W2419491452 cites W2028046413 @default.
- W2419491452 cites W2031799817 @default.
- W2419491452 cites W2041504532 @default.
- W2419491452 cites W2047560778 @default.
- W2419491452 cites W2055563809 @default.
- W2419491452 cites W2058722589 @default.
- W2419491452 cites W2065718044 @default.
- W2419491452 cites W2066713758 @default.
- W2419491452 cites W2068655098 @default.
- W2419491452 cites W2078146328 @default.
- W2419491452 cites W2085515413 @default.
- W2419491452 cites W2088282077 @default.
- W2419491452 cites W2090588433 @default.
- W2419491452 cites W2099122603 @default.
- W2419491452 cites W2111533830 @default.
- W2419491452 cites W2116351302 @default.
- W2419491452 cites W2127260166 @default.
- W2419491452 cites W2137665397 @default.
- W2419491452 cites W2143098148 @default.
- W2419491452 cites W2149951656 @default.
- W2419491452 cites W2151770991 @default.
- W2419491452 cites W2161413435 @default.
- W2419491452 cites W2219382184 @default.
- W2419491452 cites W2319663186 @default.
- W2419491452 cites W2329962099 @default.
- W2419491452 doi "https://doi.org/10.1002/jssc.201600354" @default.
- W2419491452 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27279456" @default.
- W2419491452 hasPublicationYear "2016" @default.
- W2419491452 type Work @default.
- W2419491452 sameAs 2419491452 @default.
- W2419491452 citedByCount "21" @default.
- W2419491452 countsByYear W24194914522017 @default.
- W2419491452 countsByYear W24194914522018 @default.
- W2419491452 countsByYear W24194914522019 @default.
- W2419491452 countsByYear W24194914522021 @default.
- W2419491452 countsByYear W24194914522022 @default.
- W2419491452 crossrefType "journal-article" @default.
- W2419491452 hasAuthorship W2419491452A5023572041 @default.
- W2419491452 hasAuthorship W2419491452A5031371599 @default.
- W2419491452 hasAuthorship W2419491452A5034145752 @default.
- W2419491452 hasAuthorship W2419491452A5046831186 @default.
- W2419491452 hasAuthorship W2419491452A5051465571 @default.
- W2419491452 hasAuthorship W2419491452A5062803279 @default.
- W2419491452 hasAuthorship W2419491452A5063493127 @default.
- W2419491452 hasAuthorship W2419491452A5080517520 @default.
- W2419491452 hasAuthorship W2419491452A5083083294 @default.
- W2419491452 hasConcept C113196181 @default.
- W2419491452 hasConcept C119128265 @default.
- W2419491452 hasConcept C146686406 @default.
- W2419491452 hasConcept C161790260 @default.
- W2419491452 hasConcept C162356407 @default.
- W2419491452 hasConcept C16838498 @default.
- W2419491452 hasConcept C178790620 @default.
- W2419491452 hasConcept C179998833 @default.
- W2419491452 hasConcept C185592680 @default.
- W2419491452 hasConcept C2779433975 @default.
- W2419491452 hasConcept C43617362 @default.
- W2419491452 hasConcept C486523 @default.
- W2419491452 hasConcept C73826308 @default.
- W2419491452 hasConcept C93237805 @default.
- W2419491452 hasConceptScore W2419491452C113196181 @default.
- W2419491452 hasConceptScore W2419491452C119128265 @default.
- W2419491452 hasConceptScore W2419491452C146686406 @default.
- W2419491452 hasConceptScore W2419491452C161790260 @default.
- W2419491452 hasConceptScore W2419491452C162356407 @default.
- W2419491452 hasConceptScore W2419491452C16838498 @default.
- W2419491452 hasConceptScore W2419491452C178790620 @default.
- W2419491452 hasConceptScore W2419491452C179998833 @default.
- W2419491452 hasConceptScore W2419491452C185592680 @default.
- W2419491452 hasConceptScore W2419491452C2779433975 @default.
- W2419491452 hasConceptScore W2419491452C43617362 @default.
- W2419491452 hasConceptScore W2419491452C486523 @default.
- W2419491452 hasConceptScore W2419491452C73826308 @default.
- W2419491452 hasConceptScore W2419491452C93237805 @default.