Matches in SemOpenAlex for { <https://semopenalex.org/work/W2419587251> ?p ?o ?g. }
Showing items 1 to 74 of
74
with 100 items per page.
- W2419587251 endingPage "16" @default.
- W2419587251 startingPage "1" @default.
- W2419587251 abstract "The aim of this project was to develop procedures necessary to study mechanisms of receptor mediated gene transfer by means of integrated microscopy. Plasmid DNA was incorporated into a transfection complex consisting of poly(L)lysine and transferrin to which the nuclear localization signal was conjugated. This complex was presented to cultured glioma cells. Preparation of the transfected DNA for imaging was pursued by two methods. In the first method tetramethylrhodamine, nanogold, and ferritin were linked through streptavidin to the biotinylated plasmid DNA. Trafficking of the fluorescent derivatives was studied in living cells with fluorescence microscopy. Then, selected cells were rapidly cryo-immobilized. Ultra-structural distribution of the transfected DNA was imaged with energy filtering transmission electron microscopy. In the second method, the unmodified transfected DNA was detected in cryo-immobilized cells by in situ polymerase chain reaction and in situ hybridization. For laser scanning fluorescence microscopy probes were labeled with tetramethylrhodamine. For ultrastructural analysis by electron spectroscopic imaging, probes containing incorporated digoxigenin were labeled with anti-digoxigenin boronated antibodies. Based upon the developed procedures, it has been demonstrated that the presence of the nuclear localization signal in the transfection complex resulted in rapid nuclear import of the transfected DNA." @default.
- W2419587251 created "2016-06-24" @default.
- W2419587251 creator A5055402593 @default.
- W2419587251 date "1996-01-01" @default.
- W2419587251 modified "2023-09-23" @default.
- W2419587251 title "Preparation of plasmid DNA in transfection complexes for fluorescence and electron spectroscopic imaging." @default.
- W2419587251 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9601525" @default.
- W2419587251 hasPublicationYear "1996" @default.
- W2419587251 type Work @default.
- W2419587251 sameAs 2419587251 @default.
- W2419587251 citedByCount "4" @default.
- W2419587251 countsByYear W24195872512012 @default.
- W2419587251 crossrefType "journal-article" @default.
- W2419587251 hasAuthorship W2419587251A5055402593 @default.
- W2419587251 hasConcept C104317684 @default.
- W2419587251 hasConcept C120665830 @default.
- W2419587251 hasConcept C121332964 @default.
- W2419587251 hasConcept C12554922 @default.
- W2419587251 hasConcept C130197536 @default.
- W2419587251 hasConcept C150194340 @default.
- W2419587251 hasConcept C153911025 @default.
- W2419587251 hasConcept C169274487 @default.
- W2419587251 hasConcept C185592680 @default.
- W2419587251 hasConcept C204628709 @default.
- W2419587251 hasConcept C22744801 @default.
- W2419587251 hasConcept C2776190903 @default.
- W2419587251 hasConcept C2776552150 @default.
- W2419587251 hasConcept C2778204606 @default.
- W2419587251 hasConcept C54009773 @default.
- W2419587251 hasConcept C552990157 @default.
- W2419587251 hasConcept C55493867 @default.
- W2419587251 hasConcept C81439078 @default.
- W2419587251 hasConcept C86803240 @default.
- W2419587251 hasConcept C91881484 @default.
- W2419587251 hasConceptScore W2419587251C104317684 @default.
- W2419587251 hasConceptScore W2419587251C120665830 @default.
- W2419587251 hasConceptScore W2419587251C121332964 @default.
- W2419587251 hasConceptScore W2419587251C12554922 @default.
- W2419587251 hasConceptScore W2419587251C130197536 @default.
- W2419587251 hasConceptScore W2419587251C150194340 @default.
- W2419587251 hasConceptScore W2419587251C153911025 @default.
- W2419587251 hasConceptScore W2419587251C169274487 @default.
- W2419587251 hasConceptScore W2419587251C185592680 @default.
- W2419587251 hasConceptScore W2419587251C204628709 @default.
- W2419587251 hasConceptScore W2419587251C22744801 @default.
- W2419587251 hasConceptScore W2419587251C2776190903 @default.
- W2419587251 hasConceptScore W2419587251C2776552150 @default.
- W2419587251 hasConceptScore W2419587251C2778204606 @default.
- W2419587251 hasConceptScore W2419587251C54009773 @default.
- W2419587251 hasConceptScore W2419587251C552990157 @default.
- W2419587251 hasConceptScore W2419587251C55493867 @default.
- W2419587251 hasConceptScore W2419587251C81439078 @default.
- W2419587251 hasConceptScore W2419587251C86803240 @default.
- W2419587251 hasConceptScore W2419587251C91881484 @default.
- W2419587251 hasLocation W24195872511 @default.
- W2419587251 hasOpenAccess W2419587251 @default.
- W2419587251 hasPrimaryLocation W24195872511 @default.
- W2419587251 hasRelatedWork W2002473487 @default.
- W2419587251 hasRelatedWork W2020199268 @default.
- W2419587251 hasRelatedWork W202910747 @default.
- W2419587251 hasRelatedWork W2036923488 @default.
- W2419587251 hasRelatedWork W2083193506 @default.
- W2419587251 hasRelatedWork W2089056957 @default.
- W2419587251 hasRelatedWork W2138941105 @default.
- W2419587251 hasRelatedWork W2379416539 @default.
- W2419587251 hasRelatedWork W2419587251 @default.
- W2419587251 hasRelatedWork W986195926 @default.
- W2419587251 hasVolume "10" @default.
- W2419587251 isParatext "false" @default.
- W2419587251 isRetracted "false" @default.
- W2419587251 magId "2419587251" @default.
- W2419587251 workType "article" @default.