Matches in SemOpenAlex for { <https://semopenalex.org/work/W2420565184> ?p ?o ?g. }
Showing items 1 to 74 of
74
with 100 items per page.
- W2420565184 endingPage "31" @default.
- W2420565184 startingPage "427" @default.
- W2420565184 abstract "To study the effect of substrate stiffness on proliferation, migration of fibroblast and integrin β(1) expression in fibroblast.Fibroblasts were inoculated on silicon substrate with stiffness of (16.2 ± 0.5), (19.8 ± 1.1), and (200.1 ± 2.6) kPa. After being cultured for 5 days or 6 days, cells were counted and cell proliferative activities (recorded as absorbance value) were assessed with methyl thiazolyl blue (MTT). After being cultured for 3 days, cell cycle was detected and proliferation index (PI) was calculated. The cell scratch test was used for determination of cell migration rate on post scratch day (PSD) 0 (the day of scratch), 1, 2, and 3. After being cultured for 2 days, the expression of integrin β(1) was determined by flow cytometry with fluorescence. Data were processed with one-way analysis of variance.(1) The proliferative speed and proliferative activity of fibroblasts were all increased along with the increase in substrate stiffness. PI of fibroblasts inoculated on silicon substrate with stiffness of (16.2 ± 0.5), (19.8 ± 1.1), and (200.1 ± 2.6) kPa was respectively 24.8%, 27.4%, 32.4%. On PSD 2, migration rate of fibroblasts inoculated on silicon substrate with stiffness of (19.8 ± 1.1) and (200.1 ± 2.6) kPa was respectively (91.4 ± 5.1)%, (100.0 ± 1.3)%, which were higher than that of fibroblasts inoculated on silicon substrate with stiffness of (16.2 ± 0.5) kPa [(55.8 ± 6.8)%, with F value respectively 3.5, 4.0, P values all below 0.01]. (3) The expression rate of integrin β(1) in fibroblasts inoculated on silicon substrate with stiffness of (16.2 ± 0.5) kPa was the lowest (43.22%), and that in fibroblast inoculated on silicon substrate with stiffness of (200.1 ± 2.6) kPa was the highest (81.26%).Substrate stiffness may have a great effect on proliferation and migration of fibroblast during the process of wound healing and scar formation, which can be related to regulation of integrin β(1) expression." @default.
- W2420565184 created "2016-06-24" @default.
- W2420565184 creator A5008204431 @default.
- W2420565184 creator A5031039660 @default.
- W2420565184 creator A5076491707 @default.
- W2420565184 creator A5079594267 @default.
- W2420565184 date "2011-12-01" @default.
- W2420565184 modified "2023-10-18" @default.
- W2420565184 title "[Effect of substrate stiffness on biological behavior of fibroblasts]." @default.
- W2420565184 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22340788" @default.
- W2420565184 hasPublicationYear "2011" @default.
- W2420565184 type Work @default.
- W2420565184 sameAs 2420565184 @default.
- W2420565184 citedByCount "0" @default.
- W2420565184 crossrefType "journal-article" @default.
- W2420565184 hasAuthorship W2420565184A5008204431 @default.
- W2420565184 hasAuthorship W2420565184A5031039660 @default.
- W2420565184 hasAuthorship W2420565184A5076491707 @default.
- W2420565184 hasAuthorship W2420565184A5079594267 @default.
- W2420565184 hasConcept C12554922 @default.
- W2420565184 hasConcept C1491633281 @default.
- W2420565184 hasConcept C153911025 @default.
- W2420565184 hasConcept C159985019 @default.
- W2420565184 hasConcept C185592680 @default.
- W2420565184 hasConcept C18903297 @default.
- W2420565184 hasConcept C192562407 @default.
- W2420565184 hasConcept C202751555 @default.
- W2420565184 hasConcept C2777289219 @default.
- W2420565184 hasConcept C2780381497 @default.
- W2420565184 hasConcept C54355233 @default.
- W2420565184 hasConcept C553184892 @default.
- W2420565184 hasConcept C55493867 @default.
- W2420565184 hasConcept C62112901 @default.
- W2420565184 hasConcept C81885089 @default.
- W2420565184 hasConcept C84416704 @default.
- W2420565184 hasConcept C86803240 @default.
- W2420565184 hasConceptScore W2420565184C12554922 @default.
- W2420565184 hasConceptScore W2420565184C1491633281 @default.
- W2420565184 hasConceptScore W2420565184C153911025 @default.
- W2420565184 hasConceptScore W2420565184C159985019 @default.
- W2420565184 hasConceptScore W2420565184C185592680 @default.
- W2420565184 hasConceptScore W2420565184C18903297 @default.
- W2420565184 hasConceptScore W2420565184C192562407 @default.
- W2420565184 hasConceptScore W2420565184C202751555 @default.
- W2420565184 hasConceptScore W2420565184C2777289219 @default.
- W2420565184 hasConceptScore W2420565184C2780381497 @default.
- W2420565184 hasConceptScore W2420565184C54355233 @default.
- W2420565184 hasConceptScore W2420565184C553184892 @default.
- W2420565184 hasConceptScore W2420565184C55493867 @default.
- W2420565184 hasConceptScore W2420565184C62112901 @default.
- W2420565184 hasConceptScore W2420565184C81885089 @default.
- W2420565184 hasConceptScore W2420565184C84416704 @default.
- W2420565184 hasConceptScore W2420565184C86803240 @default.
- W2420565184 hasIssue "6" @default.
- W2420565184 hasLocation W24205651841 @default.
- W2420565184 hasOpenAccess W2420565184 @default.
- W2420565184 hasPrimaryLocation W24205651841 @default.
- W2420565184 hasRelatedWork W2060319409 @default.
- W2420565184 hasRelatedWork W2355897695 @default.
- W2420565184 hasRelatedWork W2362905450 @default.
- W2420565184 hasRelatedWork W2363589313 @default.
- W2420565184 hasRelatedWork W2364535361 @default.
- W2420565184 hasRelatedWork W2374592133 @default.
- W2420565184 hasRelatedWork W2376135799 @default.
- W2420565184 hasRelatedWork W2387175565 @default.
- W2420565184 hasRelatedWork W2424559734 @default.
- W2420565184 hasRelatedWork W4291127362 @default.
- W2420565184 hasVolume "27" @default.
- W2420565184 isParatext "false" @default.
- W2420565184 isRetracted "false" @default.
- W2420565184 magId "2420565184" @default.
- W2420565184 workType "article" @default.