FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2421007310> ?p ?o ?g. }

• W2421007310 endingPage "8" @default.
• W2421007310 startingPage "932" @default.
• W2421007310 abstract "The multidrug resistance gene, MDR1, is one of the genes responsible for resistance to chemotherapy in the treatment of leukaemia and other cancers. The discovery of RNA interference in mammalian cells has provided a powerful tool to inhibit the expression of this gene. However, very little is known about the transfection of leukaemia cells with short interfering RNA (siRNA) targeted at MDR1. This study aims to evaluate the effectiveness of two chemically-synthesised siRNA in modulating MDR1 gene and inhibiting P-glycoprotein expression in leukaemic cells. We also evaluated two siRNA delivery methods in this study.K562/Adr was transfected with two MDR1-targeted siRNA or negative control siRNA, by using cationic lipid-based transfection reagents or electroporator. Gene expression of MDR1 was quantified by real-time polymerase chain reaction and calculated as a percentage relative to the negative control siRNA. P-glycoprotein expression was evaluated via flow cytometry and drug sensitivity after treatment was assessed by cytotoxicity assays.The percentage of MDR1 gene knockdown from cells transfected with an electroporator was significantly higher (84.4 percent, p-value is 0.094) compared to cells transfected with cationic lipid-based transfection reagents (52.8 percent). Both siRNA significantly reduced the expression of MDR1 by 84.9 percent (p-value is 0.001) and 86.0 percent (p-value is 0.011), respectively. P-glycoprotein expression was down-regulated and drug sensitivity was increased after treatment with the siRNA.This study shows that the two siRNA sequences are capable of modulating MDR1 and P-glycoprotein expressions and increased drug sensitivity. Transfection with an electroporator was superior to chemical transfection for leukaemia cells." @default.
• W2421007310 created "2016-06-24" @default.
• W2421007310 creator A5000263886 @default.
• W2421007310 creator A5022265572 @default.
• W2421007310 creator A5036315007 @default.
• W2421007310 creator A5039936008 @default.
• W2421007310 creator A5040830222 @default.
• W2421007310 date "2007-10-01" @default.
• W2421007310 modified "2023-09-23" @default.
• W2421007310 title "Modulating multidrug resistance gene in leukaemia cells by short interfering RNA." @default.
• W2421007310 cites W1505265524 @default.
• W2421007310 cites W1510962977 @default.
• W2421007310 cites W1604521649 @default.
• W2421007310 cites W1754065147 @default.
• W2421007310 cites W1958840615 @default.
• W2421007310 cites W1968931590 @default.
• W2421007310 cites W1974155519 @default.
• W2421007310 cites W1978993203 @default.
• W2421007310 cites W1991664367 @default.
• W2421007310 cites W1994642517 @default.
• W2421007310 cites W2007607838 @default.
• W2421007310 cites W2013968494 @default.
• W2421007310 cites W2037930879 @default.
• W2421007310 cites W2050767064 @default.
• W2421007310 cites W2056183399 @default.
• W2421007310 cites W2068562307 @default.
• W2421007310 cites W2083263885 @default.
• W2421007310 cites W2088419541 @default.
• W2421007310 cites W2094341952 @default.
• W2421007310 cites W2095108054 @default.
• W2421007310 cites W2099249565 @default.
• W2421007310 cites W2110976740 @default.
• W2421007310 cites W2118234018 @default.
• W2421007310 cites W2136447541 @default.
• W2421007310 cites W2149320251 @default.
• W2421007310 cites W2163678477 @default.
• W2421007310 cites W2230711222 @default.
• W2421007310 cites W2266572268 @default.
• W2421007310 cites W2337432642 @default.
• W2421007310 cites W2402221242 @default.
• W2421007310 cites W2764775166 @default.
• W2421007310 cites W329750643 @default.
• W2421007310 cites W61975600 @default.
• W2421007310 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17909680" @default.
• W2421007310 hasPublicationYear "2007" @default.
• W2421007310 type Work @default.
• W2421007310 sameAs 2421007310 @default.
• W2421007310 citedByCount "3" @default.
• W2421007310 crossrefType "journal-article" @default.
• W2421007310 hasAuthorship W2421007310A5000263886 @default.
• W2421007310 hasAuthorship W2421007310A5022265572 @default.
• W2421007310 hasAuthorship W2421007310A5036315007 @default.
• W2421007310 hasAuthorship W2421007310A5039936008 @default.
• W2421007310 hasAuthorship W2421007310A5040830222 @default.
• W2421007310 hasConcept C104317684 @default.
• W2421007310 hasConcept C109316439 @default.
• W2421007310 hasConcept C114851261 @default.
• W2421007310 hasConcept C133936738 @default.
• W2421007310 hasConcept C150194340 @default.
• W2421007310 hasConcept C153911025 @default.
• W2421007310 hasConcept C166703698 @default.
• W2421007310 hasConcept C171122931 @default.
• W2421007310 hasConcept C173396325 @default.
• W2421007310 hasConcept C202751555 @default.
• W2421007310 hasConcept C22615655 @default.
• W2421007310 hasConcept C2778707650 @default.
• W2421007310 hasConcept C502942594 @default.
• W2421007310 hasConcept C54009773 @default.
• W2421007310 hasConcept C54355233 @default.
• W2421007310 hasConcept C553184892 @default.
• W2421007310 hasConcept C55493867 @default.
• W2421007310 hasConcept C67705224 @default.
• W2421007310 hasConcept C71924100 @default.
• W2421007310 hasConcept C86803240 @default.
• W2421007310 hasConceptScore W2421007310C104317684 @default.
• W2421007310 hasConceptScore W2421007310C109316439 @default.
• W2421007310 hasConceptScore W2421007310C114851261 @default.
• W2421007310 hasConceptScore W2421007310C133936738 @default.
• W2421007310 hasConceptScore W2421007310C150194340 @default.
• W2421007310 hasConceptScore W2421007310C153911025 @default.
• W2421007310 hasConceptScore W2421007310C166703698 @default.
• W2421007310 hasConceptScore W2421007310C171122931 @default.
• W2421007310 hasConceptScore W2421007310C173396325 @default.
• W2421007310 hasConceptScore W2421007310C202751555 @default.
• W2421007310 hasConceptScore W2421007310C22615655 @default.
• W2421007310 hasConceptScore W2421007310C2778707650 @default.
• W2421007310 hasConceptScore W2421007310C502942594 @default.
• W2421007310 hasConceptScore W2421007310C54009773 @default.
• W2421007310 hasConceptScore W2421007310C54355233 @default.
• W2421007310 hasConceptScore W2421007310C553184892 @default.
• W2421007310 hasConceptScore W2421007310C55493867 @default.
• W2421007310 hasConceptScore W2421007310C67705224 @default.
• W2421007310 hasConceptScore W2421007310C71924100 @default.
• W2421007310 hasConceptScore W2421007310C86803240 @default.
• W2421007310 hasIssue "10" @default.
• W2421007310 hasLocation W24210073101 @default.
• W2421007310 hasOpenAccess W2421007310 @default.
• W2421007310 hasPrimaryLocation W24210073101 @default.

• next