Matches in SemOpenAlex for { <https://semopenalex.org/work/W2421055934> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W2421055934 endingPage "1564" @default.
- W2421055934 startingPage "1559" @default.
- W2421055934 abstract "Objective To observe the effects of S-adenosylmethionine (SAM) on cell proliferation, cell cycles, apoptosis and invasive capacity of gastric cancer cell lines SGC-7901 and BGC-823 and detect the methylation status and expression of c-myc and urokinase-type plasminogen activator ( uPA). Methods The effect of SAM on proliferation of SGC-7901 and BGC-823 cells were determined by MTT assay. SGC- 7901 and BGC-823 cells were treated with different concentrations of SAM(0, 2, 4 mmol/L)for 72 h. Then flow cytometry was used to detect the change of cell cycles and apoptosis; Transwell assay to detect the invasion; RT-PCR and Western blot to detect the expression of c-myc and uPA; and MSP to detect the methylation of c-myc and uPA. Results SAM displayed a growth-inhibiting effect on SGC-7901 and BGC- 823 cells in a dose- and time-dependent manner after exposure to SAM at different concentrations (0. 5±32 mmol/L) for 24, 48 and 72 h, cell proliferation were significantly restrained (all P <0. 05); 72 h IC50 SGC- 7901 5.40 mmol/L and BGC-823 4.01 mmol/L After treating SGC-7901 and BGC-823 with different concentrations of SAM, the cell percentages of G0/G1 phase significantly increased ( P < 0. 05 and P < 0.01) while the cell proliferation indices significantly decreased (P<0. 05 and P<0. 01). Compared with control group(0. 33 ±0. 09), the cell apoptosis of 2 mmol/L(5. 79 ±0. 75)and 4 mmol/L groups( 10. 19 ± 0. 60) of SGC-7901 were obviously reduced (all P <0. 01). Compared with control group (0. 95±0. 19), the cell apoptosis of 2 mmol/L (6. 23±0. 75 ) and 4 mmol/L groups (11. 82±1.14) of BGC-823 were obviously reduced (all P <0. 01). The cell invasive capacity were significantly restrained (P <0. 01 ). The invasion inhibition ratio of 2 mmol/L and 4 mmol/L groups of SGC-7901 were 51.07 % and 80.69% respectively. The invasion inhibition ratio of 2 mmol/L and 4 mmol/L groups of BGC-823 were 48. 57% and 84. 10% respectively. The expressions of c-myc and uPA significantly decreased (P <0.05 and P <0. 01). There was no expression of c-myc in 2 mmol/L group of BGC-823. The methylation of c-myc and uPA genes in two cell lines were reversed after SAM treatment. Conclusions SAM can induce the apoptosis of SGC-7901 and BGC-823, block the cell cycles at G0/G1 phase and suppress the proliferation and invasion of these two cell lines. SAM can reverse the methylation of c-myc and uPA in these two cell lines and reduce their expression. SAM may act as a methyl donor to restrain the development and progression of tumor when hypomethylation is widely present in cancer.Key words: S-adenosylmethionine; Stomach neoplasms; DNA methylation" @default.
- W2421055934 created "2016-06-24" @default.
- W2421055934 creator A5010777233 @default.
- W2421055934 creator A5015741830 @default.
- W2421055934 creator A5036913519 @default.
- W2421055934 creator A5062203269 @default.
- W2421055934 creator A5083607904 @default.
- W2421055934 creator A5086934712 @default.
- W2421055934 date "2010-06-08" @default.
- W2421055934 modified "2023-10-17" @default.
- W2421055934 title "[Effects of S-adenosylmethionine on gastric cancer cell lines SGC-7901 and BGC-823]" @default.
- W2421055934 doi "https://doi.org/10.3760/cma.j.issn.0376-2491.2010.22.013" @default.
- W2421055934 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20973239" @default.
- W2421055934 hasPublicationYear "2010" @default.
- W2421055934 type Work @default.
- W2421055934 sameAs 2421055934 @default.
- W2421055934 citedByCount "0" @default.
- W2421055934 crossrefType "journal-article" @default.
- W2421055934 hasAuthorship W2421055934A5010777233 @default.
- W2421055934 hasAuthorship W2421055934A5015741830 @default.
- W2421055934 hasAuthorship W2421055934A5036913519 @default.
- W2421055934 hasAuthorship W2421055934A5062203269 @default.
- W2421055934 hasAuthorship W2421055934A5083607904 @default.
- W2421055934 hasAuthorship W2421055934A5086934712 @default.
- W2421055934 hasConcept C104317684 @default.
- W2421055934 hasConcept C1491633281 @default.
- W2421055934 hasConcept C153911025 @default.
- W2421055934 hasConcept C185592680 @default.
- W2421055934 hasConcept C190283241 @default.
- W2421055934 hasConcept C2583947 @default.
- W2421055934 hasConcept C2776415932 @default.
- W2421055934 hasConcept C2780783641 @default.
- W2421055934 hasConcept C29537977 @default.
- W2421055934 hasConcept C33288867 @default.
- W2421055934 hasConcept C54355233 @default.
- W2421055934 hasConcept C553184892 @default.
- W2421055934 hasConcept C55493867 @default.
- W2421055934 hasConcept C62112901 @default.
- W2421055934 hasConcept C81885089 @default.
- W2421055934 hasConcept C86803240 @default.
- W2421055934 hasConceptScore W2421055934C104317684 @default.
- W2421055934 hasConceptScore W2421055934C1491633281 @default.
- W2421055934 hasConceptScore W2421055934C153911025 @default.
- W2421055934 hasConceptScore W2421055934C185592680 @default.
- W2421055934 hasConceptScore W2421055934C190283241 @default.
- W2421055934 hasConceptScore W2421055934C2583947 @default.
- W2421055934 hasConceptScore W2421055934C2776415932 @default.
- W2421055934 hasConceptScore W2421055934C2780783641 @default.
- W2421055934 hasConceptScore W2421055934C29537977 @default.
- W2421055934 hasConceptScore W2421055934C33288867 @default.
- W2421055934 hasConceptScore W2421055934C54355233 @default.
- W2421055934 hasConceptScore W2421055934C553184892 @default.
- W2421055934 hasConceptScore W2421055934C55493867 @default.
- W2421055934 hasConceptScore W2421055934C62112901 @default.
- W2421055934 hasConceptScore W2421055934C81885089 @default.
- W2421055934 hasConceptScore W2421055934C86803240 @default.
- W2421055934 hasIssue "22" @default.
- W2421055934 hasLocation W24210559341 @default.
- W2421055934 hasLocation W24210559342 @default.
- W2421055934 hasOpenAccess W2421055934 @default.
- W2421055934 hasPrimaryLocation W24210559341 @default.
- W2421055934 hasRelatedWork W2353250633 @default.
- W2421055934 hasRelatedWork W2359680295 @default.
- W2421055934 hasRelatedWork W2361634539 @default.
- W2421055934 hasRelatedWork W2363347650 @default.
- W2421055934 hasRelatedWork W2369774475 @default.
- W2421055934 hasRelatedWork W2371899026 @default.
- W2421055934 hasRelatedWork W2378921952 @default.
- W2421055934 hasRelatedWork W2383820488 @default.
- W2421055934 hasRelatedWork W2384060620 @default.
- W2421055934 hasRelatedWork W2394298293 @default.
- W2421055934 hasRelatedWork W2420795765 @default.
- W2421055934 hasRelatedWork W2921146212 @default.
- W2421055934 hasRelatedWork W3028616425 @default.
- W2421055934 hasRelatedWork W3030092310 @default.
- W2421055934 hasRelatedWork W3031274914 @default.
- W2421055934 hasRelatedWork W3031862913 @default.
- W2421055934 hasRelatedWork W3032007681 @default.
- W2421055934 hasRelatedWork W3032198128 @default.
- W2421055934 hasRelatedWork W3032235088 @default.
- W2421055934 hasRelatedWork W3139316228 @default.
- W2421055934 hasVolume "90" @default.
- W2421055934 isParatext "false" @default.
- W2421055934 isRetracted "false" @default.
- W2421055934 magId "2421055934" @default.
- W2421055934 workType "article" @default.