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- W2421089341 abstract "The capacity of a primary hepatocyte culture of black catfish (Ictalurus melas) to metabolise toxic compounds was evaluated by using aflatoxin B 1 (AFB 1 ) as a xenobiotic model. Hepatocytes were isolated by a two-step liver perfusion procedure and cultured in a serum-free medium on both porous membranes and 96-well microplates coated with Matrigel®. A viability of 95–98% was obtained. The ability to maintain cytochrome P450 activities was examined by measuring ethoxyresorufin- O-deethylase and pentoxyresorufin- O-depentylase activities, at various culture times. The cytotoxic effects of AFB 1 on catfish hepatocytes were examined by using a neutral red uptake assay on 96-well microplates. The concentration causing 50% inhibition of uptake (IC50) value was 3μM. The AFB 1 metabolites were analysed by HPLC with an ultraviolet diode array and fluorescent detectors. The results obtained show that catfish hepatocytes cultured on Matrigel retain their AFB 1 metabolising activities for at least 6 days after seeding. This comparative study, using primary hepatocytes and isolated microsomes from black catfish, trout and mice, confirms that the observed marked differences in toxic response following exposure to AFB 1 in these species are likely to be related to their various metabolising capacities." @default.
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- W2421089341 date "1998-03-01" @default.
- W2421089341 modified "2023-09-27" @default.
- W2421089341 title "In VitroStudies on the Metabolism and Toxicity of Aflatoxin B1in Primary Cultures of Black Catfish(Ictalurus melas)Hepatocytes" @default.
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- W2421089341 doi "https://doi.org/10.1177/026119299802600207" @default.
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