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• W2421296043 abstract "Multiplex quantitative polymerase chain reaction (PCR) based on novel design of fluorescent primers is described. Self-quenched fluorogenic primers are labeled with a single fluorophore on a base close to the 3'-end with no quencher required. A tail of 5-7 nucleotides is added to the 5'-end of the primer to form a blunt-end hairpin when the primer is not incorporated into a PCR product. This design provides a low initial fluorescence of the primers that increases upon formation of the PCR product. The hairpin oligonucleotides (DeltaG from -1.6 to -5.8 kcal/mol) are as efficient as linear primers and provide additional specificity to the PCR by preventing primer-dimers and mispriming. Self-quenched primers could be designed manually or by specialized software and could be used for real-time gene quantitation. Targets of 10-107 copies could be detected with precision in PCR using fluorescein-labeled primers for variable genes and JOE-labeled primers for the reference genes. This method could also be used to detect single nucleotide polymorphism by allele-specific PCR. In conclusion, self-quenched primers are an efficient and cost-effective alternative to fluorescence resonance energy transfer-labeled oligonucleotides." @default.
• W2421296043 created "2016-06-24" @default.
• W2421296043 creator A5045031133 @default.
• W2421296043 date "2006-06-24" @default.
• W2421296043 modified "2023-09-25" @default.
• W2421296043 title "Homogeneous Detection of Nucleic Acids Using Self-Quenched Polymerase Chain Reaction Primers Labeled With a Single Fluorophore (LUX™ Primers)" @default.
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• W2421296043 doi "https://doi.org/10.1385/1-59745-069-3:95" @default.
• W2421296043 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16785623" @default.
• W2421296043 hasPublicationYear "2006" @default.
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