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• W2421790520 abstract "To understand mechanobiology, a quantitative understanding of how cells interact mechanically with their environment is needed. Cell mechanics is important to study as they play a role in cell behaviors ranging from cell signaling to epithelial to mesenchymal transition in physiological processes such as development and cancer. To study changes in cell contractile behavior, numerous quantitative measurement techniques have been developed based on the measurement of deformations of a substrate from an initial state. Herein, we present details on a technique we have developed for the measurements of 2D cellular traction forces with the goal of facilitating adaptation of this technique by other investigators. This technique is flexible in that it utilizes well-studied methods for microcontact printing and fabrication of polyacrylamide hydrogels to generate regular arrays of patterns that can be transferred onto the hydrogels. From the deformation of the arrays, an automated algorithm can be used to quantitatively determine the traction forces exerted by the cells onto the adhesion points. The simplicity and flexibility of this technique make it a useful contribution to our toolbox for measurement of cell traction forces." @default.
• W2421790520 created "2016-06-24" @default.
• W2421790520 creator A5027850294 @default.
• W2421790520 creator A5052657682 @default.
• W2421790520 date "2014-01-01" @default.
• W2421790520 modified "2023-10-17" @default.
• W2421790520 title "Patterned Hydrogels for Simplified Measurement of Cell Traction Forces" @default.
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• W2421790520 doi "https://doi.org/10.1016/b978-0-12-800281-0.00002-6" @default.
• W2421790520 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24560500" @default.
• W2421790520 hasPublicationYear "2014" @default.
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