Matches in SemOpenAlex for { <https://semopenalex.org/work/W2421925466> ?p ?o ?g. }
Showing items 1 to 82 of
82
with 100 items per page.
- W2421925466 endingPage "400" @default.
- W2421925466 startingPage "397" @default.
- W2421925466 abstract "Objective To investigate the effects of NG-nitro-L-arginine (L-NA) on pulmonary surfactant (PS) and pulmonary cells apoptosis in lipopolysaccharide (LPS) induced acute lung injury (ALI). Methods Twenty-four male Sprague-Dawley (SD) rats were randomly divided into three groups: control group, model group, L-NA group. Model of ALI was reproduced by injection of LPS 5 mg/kg via sublingual vein in model group and L-NA group. L-NA (20 mg/kg) was administered in L-NA group, while normal saline was administered in control group and model group 3 hours after LPS injection. The rats were sacrificed at 6 hours after LPS injection, and the lung tissue was obtained for measuring the expressions of pulmonary surfactant protein A (SP-A) mRNA by in situ hybridization (ISH) method; meanwhile, apoptosis rate was evaluated by flow cytometry; the expression of caspase-3 was evaluated by Western blotting analysis; Bcl-2 and Bax were evaluated respectively by immunohistochemisty (IHC). Results Compared with that of the control group, SP-A mRNA [absorbance (A) value] in the lung tissue was significantly decreased by LPS (0.071±0.017 vs. 0.113±0.021) in model group, apoptosis rate of pulmonary cells [(25.04±4.57)% vs. (11.37±3.08)%], caspase-3 protein expression (A value: 298.64±37.11 vs. 110.24±14.35) and Bax protein expression (A value: 0.145±0.011 vs. 0.076±0.010) were significantly increased, Bcl-2 protein expression (A value: 0.064±0.011 vs. 0.073±0.009) and Bcl-2/Bax (0.447±0.086 vs. 0.976±0.157) were decreased in model group (all P 0.05). Conclusion L-NA had no effect on LPS-induced pulmonary cell apoptosis and had no effect on the expressions of caspase-3 and Bax, but L-NA can protect the lung from LPS-induced injury by up-regulating the expression of PS.Key words: NG-nitro-L-arginine; Pulmonary surfactant; Apoptosis; Acute lung injury; Lipopolysaccharide" @default.
- W2421925466 created "2016-06-24" @default.
- W2421925466 creator A5038259858 @default.
- W2421925466 creator A5059602238 @default.
- W2421925466 creator A5067681000 @default.
- W2421925466 date "2010-07-01" @default.
- W2421925466 modified "2023-09-22" @default.
- W2421925466 title "[Effect of NG-nitro-L-arginine on pulmonary surfactant and pulmonary apoptosis in acute lung injury induced by lipopolysaccharide]." @default.
- W2421925466 doi "https://doi.org/10.3760/cma.j.issn.1003-0603.2010.07.006" @default.
- W2421925466 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20663300" @default.
- W2421925466 hasPublicationYear "2010" @default.
- W2421925466 type Work @default.
- W2421925466 sameAs 2421925466 @default.
- W2421925466 citedByCount "0" @default.
- W2421925466 crossrefType "journal-article" @default.
- W2421925466 hasAuthorship W2421925466A5038259858 @default.
- W2421925466 hasAuthorship W2421925466A5059602238 @default.
- W2421925466 hasAuthorship W2421925466A5067681000 @default.
- W2421925466 hasConcept C126322002 @default.
- W2421925466 hasConcept C134018914 @default.
- W2421925466 hasConcept C142724271 @default.
- W2421925466 hasConcept C153911025 @default.
- W2421925466 hasConcept C16685009 @default.
- W2421925466 hasConcept C185592680 @default.
- W2421925466 hasConcept C190283241 @default.
- W2421925466 hasConcept C203014093 @default.
- W2421925466 hasConcept C2777397205 @default.
- W2421925466 hasConcept C2777714996 @default.
- W2421925466 hasConcept C2778754761 @default.
- W2421925466 hasConcept C553184892 @default.
- W2421925466 hasConcept C55493867 @default.
- W2421925466 hasConcept C58226133 @default.
- W2421925466 hasConcept C71924100 @default.
- W2421925466 hasConcept C86803240 @default.
- W2421925466 hasConceptScore W2421925466C126322002 @default.
- W2421925466 hasConceptScore W2421925466C134018914 @default.
- W2421925466 hasConceptScore W2421925466C142724271 @default.
- W2421925466 hasConceptScore W2421925466C153911025 @default.
- W2421925466 hasConceptScore W2421925466C16685009 @default.
- W2421925466 hasConceptScore W2421925466C185592680 @default.
- W2421925466 hasConceptScore W2421925466C190283241 @default.
- W2421925466 hasConceptScore W2421925466C203014093 @default.
- W2421925466 hasConceptScore W2421925466C2777397205 @default.
- W2421925466 hasConceptScore W2421925466C2777714996 @default.
- W2421925466 hasConceptScore W2421925466C2778754761 @default.
- W2421925466 hasConceptScore W2421925466C553184892 @default.
- W2421925466 hasConceptScore W2421925466C55493867 @default.
- W2421925466 hasConceptScore W2421925466C58226133 @default.
- W2421925466 hasConceptScore W2421925466C71924100 @default.
- W2421925466 hasConceptScore W2421925466C86803240 @default.
- W2421925466 hasIssue "7" @default.
- W2421925466 hasLocation W24219254661 @default.
- W2421925466 hasLocation W24219254662 @default.
- W2421925466 hasOpenAccess W2421925466 @default.
- W2421925466 hasPrimaryLocation W24219254661 @default.
- W2421925466 hasRelatedWork W2351104916 @default.
- W2421925466 hasRelatedWork W2353162466 @default.
- W2421925466 hasRelatedWork W2356370982 @default.
- W2421925466 hasRelatedWork W2361290174 @default.
- W2421925466 hasRelatedWork W2365941616 @default.
- W2421925466 hasRelatedWork W2367419010 @default.
- W2421925466 hasRelatedWork W2374569662 @default.
- W2421925466 hasRelatedWork W2383270008 @default.
- W2421925466 hasRelatedWork W2384523384 @default.
- W2421925466 hasRelatedWork W2391792168 @default.
- W2421925466 hasRelatedWork W2392380716 @default.
- W2421925466 hasRelatedWork W2573492709 @default.
- W2421925466 hasRelatedWork W2745850930 @default.
- W2421925466 hasRelatedWork W3028776319 @default.
- W2421925466 hasRelatedWork W3029401673 @default.
- W2421925466 hasRelatedWork W3029933832 @default.
- W2421925466 hasRelatedWork W3031106749 @default.
- W2421925466 hasRelatedWork W3031779029 @default.
- W2421925466 hasRelatedWork W3031833093 @default.
- W2421925466 hasRelatedWork W3032535797 @default.
- W2421925466 hasVolume "22" @default.
- W2421925466 isParatext "false" @default.
- W2421925466 isRetracted "false" @default.
- W2421925466 magId "2421925466" @default.
- W2421925466 workType "article" @default.