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- W2422872914 abstract "Because urinary C-reactive protein (CRP) has potential as a diagnostic marker, a sensitive enzyme immunoassay has been developed to measure this substance. This assay involves immobilizing antibody to CRP on a plastic surface, followed sequentially by addition of sample to be assayed, anti-CRP labeled with biotin, avidin conjugated with enzyme, and substrate. As little as 0.5 ng of CRP can be detected by the assay, which is reproducible and correlates well with a radial immunodiffusion method. Because the assay is inhibited by urine samples having a low pH or high urea concentration, it is necessary to pretreat the samples by solvent exchange in a commercial, disposable concentration unit. Application of the assay to clinical specimens gives results similar to those obtained previously by a complement fixation assay." @default.
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- W2422872914 date "1983-01-01" @default.
- W2422872914 modified "2023-09-23" @default.
- W2422872914 title "An enzyme immunoassay for urinary C-reactive protein." @default.
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