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• W2425182503 abstract "We present a novel strategy for the connection of phenotype and genotype in vitro that can be used for the selection of functional proteins. The strategy involves the generation of a stable complex among a ribosome, an messenger RNA and its translated protein, without removal of the termination codon, as a result of the action of the ricin A chain during translation. The technique requires no transfection, no chemical synthesis, no ligation, and no removal of the termination codon. Thus, our novel ribosome-inactivation display system should provide, without loss of the pool population, a reliable, simple, and robust selection system for the in vitro evolution of the properties of proteins in a predictable direction by a combination of randomization and appropriate selection strategies." @default.
• W2425182503 created "2016-06-24" @default.
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• W2425182503 date "2006-10-14" @default.
• W2425182503 modified "2023-10-16" @default.
• W2425182503 title "Ribosome-Inactivation Display System" @default.
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• W2425182503 doi "https://doi.org/10.1385/1-59745-187-8:221" @default.
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