Matches in SemOpenAlex for { <https://semopenalex.org/work/W2425791497> ?p ?o ?g. }
Showing items 1 to 72 of
72
with 100 items per page.
- W2425791497 endingPage "77" @default.
- W2425791497 startingPage "68" @default.
- W2425791497 abstract "To study the active sites of N-acetylhexosamine 1-kinase (NahK) from Bifidobacterium longum JCM12 17.We obtained expression strains of 10 single-mutants at 4 sites of NahK by site-directed mutagenesis, and expressed and purified both wild-type (WT) and mutant enzymes. Then, their optimum pH and optimum concentration of Mg²⁺ were determined by DNS assay and NADH-coupled microplate photometric assay, and their kinetic parameters were measured.Four mutants (D208A, D208N, D208E and I24A) lost most part of the catalytic activity. The optimum pH of mutants H31A, H31V, F247A and I24V switched from pH 7.5 (for WT) to pH 7.0, and the optimum concentration of Mg²⁺ of mutants H31A and F247A increased to 10 mmol/L from 5 mmol/L (for WT). The kinetic parameters of WT and mutants indicate that mutant F247Y had higher enzymatic activity toward GlcNAc, GalNAc and ATP than WT.The key amino acids that affect the catalytic activity of NahK were determined by site-directed mutagenesis, and together with the mutant that has higher catalytic efficiency, this has laid a foundation for further modification and evolution of NahK." @default.
- W2425791497 created "2016-06-24" @default.
- W2425791497 creator A5001827932 @default.
- W2425791497 creator A5067337421 @default.
- W2425791497 creator A5072356869 @default.
- W2425791497 creator A5073667415 @default.
- W2425791497 date "2016-01-04" @default.
- W2425791497 modified "2023-09-27" @default.
- W2425791497 title "[Active sites of N-acetylhexosamine 1-kinase from Bifidobacterium longum]." @default.
- W2425791497 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27305781" @default.
- W2425791497 hasPublicationYear "2016" @default.
- W2425791497 type Work @default.
- W2425791497 sameAs 2425791497 @default.
- W2425791497 citedByCount "0" @default.
- W2425791497 crossrefType "journal-article" @default.
- W2425791497 hasAuthorship W2425791497A5001827932 @default.
- W2425791497 hasAuthorship W2425791497A5067337421 @default.
- W2425791497 hasAuthorship W2425791497A5072356869 @default.
- W2425791497 hasAuthorship W2425791497A5073667415 @default.
- W2425791497 hasConcept C100544194 @default.
- W2425791497 hasConcept C103408237 @default.
- W2425791497 hasConcept C104317684 @default.
- W2425791497 hasConcept C143065580 @default.
- W2425791497 hasConcept C153911025 @default.
- W2425791497 hasConcept C16318435 @default.
- W2425791497 hasConcept C181199279 @default.
- W2425791497 hasConcept C185592680 @default.
- W2425791497 hasConcept C207583985 @default.
- W2425791497 hasConcept C2776996202 @default.
- W2425791497 hasConcept C2780206646 @default.
- W2425791497 hasConcept C2780305828 @default.
- W2425791497 hasConcept C41183919 @default.
- W2425791497 hasConcept C55493867 @default.
- W2425791497 hasConcept C71240020 @default.
- W2425791497 hasConcept C86803240 @default.
- W2425791497 hasConceptScore W2425791497C100544194 @default.
- W2425791497 hasConceptScore W2425791497C103408237 @default.
- W2425791497 hasConceptScore W2425791497C104317684 @default.
- W2425791497 hasConceptScore W2425791497C143065580 @default.
- W2425791497 hasConceptScore W2425791497C153911025 @default.
- W2425791497 hasConceptScore W2425791497C16318435 @default.
- W2425791497 hasConceptScore W2425791497C181199279 @default.
- W2425791497 hasConceptScore W2425791497C185592680 @default.
- W2425791497 hasConceptScore W2425791497C207583985 @default.
- W2425791497 hasConceptScore W2425791497C2776996202 @default.
- W2425791497 hasConceptScore W2425791497C2780206646 @default.
- W2425791497 hasConceptScore W2425791497C2780305828 @default.
- W2425791497 hasConceptScore W2425791497C41183919 @default.
- W2425791497 hasConceptScore W2425791497C55493867 @default.
- W2425791497 hasConceptScore W2425791497C71240020 @default.
- W2425791497 hasConceptScore W2425791497C86803240 @default.
- W2425791497 hasIssue "1" @default.
- W2425791497 hasLocation W24257914971 @default.
- W2425791497 hasOpenAccess W2425791497 @default.
- W2425791497 hasPrimaryLocation W24257914971 @default.
- W2425791497 hasRelatedWork W2013630470 @default.
- W2425791497 hasRelatedWork W2031367762 @default.
- W2425791497 hasRelatedWork W2070681424 @default.
- W2425791497 hasRelatedWork W2079312629 @default.
- W2425791497 hasRelatedWork W2124597856 @default.
- W2425791497 hasRelatedWork W2147494701 @default.
- W2425791497 hasRelatedWork W2279156773 @default.
- W2425791497 hasRelatedWork W2425791497 @default.
- W2425791497 hasRelatedWork W258580054 @default.
- W2425791497 hasRelatedWork W2792698313 @default.
- W2425791497 hasVolume "56" @default.
- W2425791497 isParatext "false" @default.
- W2425791497 isRetracted "false" @default.
- W2425791497 magId "2425791497" @default.
- W2425791497 workType "article" @default.