FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2425853225> ?p ?o ?g. }

• W2425853225 abstract "The effect of stressors, including heavy metal ions such as Cu2+, promotes activation of free radical processes in the cells of microorganisms, which causes changes in their physiological and biochemical properties and the structure of bacterial membranes. The aim of this work was to assess the influence of copper (II) sulphate on intensity of lipid peroxidation (LPO) of Chlorobium limicola IMV K-8 by measuring the content of primary (conjugated dienes and lipid hydroperoxides) and secondary lipid peroxidation products (TBA-reactive products). Microorganisms were cultivated at a temperature of 28 °C in GSB cultivation medium with exposure to light of wavelength 700–800 nm and intensity 40 lux. A suspension of C. limicola ІМV К-8 cells in the phase of exponential growth was treated for one hour with metal salt solution in concentrations 0.05–0.50 mM for investigation of the influence of copper (II) sulphate on its physiological and biochemical properties. The control samples did not contain any copper (II) sulphate. Biomass was determined by turbidity of diluted cell suspension by application of photoelectric colorimeter KFK-3. A mixture of n-heptane and isopropyl alcohol was added into cell-free extract for conjugated dienes determination. The samples were incubated at room temperature and centrifuged. Water was added into the supernatant and the samples were stirred. Ethanol was added to the heptanes phase and adsorption was measured at 233 nm. The content of lipid hydroperoxides was determined by a method based on protein precipitation by trichloroacetic acid followed by addition of ammonium thiocyanate. The concentration of TBA-reactive products in the cell-free extracts was determined by color reaction with malondialdehyde and thiobarbituric acid exposed to high temperature and acidity of the medium, which causes formation of trimetinic adduct with maximal absorption at 532 nm. It was shown that when CuSO4 was added to the incubation medium the content of conjugated dienes and lipid hydroperoxides increased with the enhancement of salt concentration. However, its value decreased by the seventh and eighth days of cultivation. The content of TBA-reactive products under the influence of copper (II) sulphate varied depending on the duration of cultivation and concentration of the metal. Its highest quantity was observed on the eighth day of cultivation. Thus it was determined that under the influence of CuSO4 the content of conjugated dienes, lipid hydroperoxides and TBA-reactive products increases. This indicates the increased activity of lipid peroxidation processes and the free radical chain reaction damage mechanism to lipids under these conditions." @default.
• W2425853225 created "2016-06-24" @default.
• W2425853225 creator A5031406937 @default.
• W2425853225 creator A5054991165 @default.
• W2425853225 creator A5066583121 @default.
• W2425853225 date "2015-12-20" @default.
• W2425853225 modified "2023-09-24" @default.
• W2425853225 title "The processes of lipid peroxidation in the cells of Chlorobium limicola IMV K-8 under the influence of copper (II) sulphate" @default.
• W2425853225 doi "https://doi.org/10.15421/011608" @default.
• W2425853225 hasPublicationYear "2015" @default.
• W2425853225 type Work @default.
• W2425853225 sameAs 2425853225 @default.
• W2425853225 citedByCount "1" @default.
• W2425853225 countsByYear W24258532252020 @default.
• W2425853225 crossrefType "journal-article" @default.
• W2425853225 hasAuthorship W2425853225A5031406937 @default.
• W2425853225 hasAuthorship W2425853225A5054991165 @default.
• W2425853225 hasAuthorship W2425853225A5066583121 @default.
• W2425853225 hasBestOaLocation W24258532251 @default.
• W2425853225 hasConcept C13965031 @default.
• W2425853225 hasConcept C178790620 @default.
• W2425853225 hasConcept C179104552 @default.
• W2425853225 hasConcept C185592680 @default.
• W2425853225 hasConcept C2778004101 @default.
• W2425853225 hasConcept C2780829032 @default.
• W2425853225 hasConcept C43617362 @default.
• W2425853225 hasConcept C544778455 @default.
• W2425853225 hasConcept C55493867 @default.
• W2425853225 hasConceptScore W2425853225C13965031 @default.
• W2425853225 hasConceptScore W2425853225C178790620 @default.
• W2425853225 hasConceptScore W2425853225C179104552 @default.
• W2425853225 hasConceptScore W2425853225C185592680 @default.
• W2425853225 hasConceptScore W2425853225C2778004101 @default.
• W2425853225 hasConceptScore W2425853225C2780829032 @default.
• W2425853225 hasConceptScore W2425853225C43617362 @default.
• W2425853225 hasConceptScore W2425853225C544778455 @default.
• W2425853225 hasConceptScore W2425853225C55493867 @default.
• W2425853225 hasLocation W24258532251 @default.
• W2425853225 hasLocation W24258532252 @default.
• W2425853225 hasOpenAccess W2425853225 @default.
• W2425853225 hasPrimaryLocation W24258532251 @default.
• W2425853225 hasRelatedWork W1973258066 @default.
• W2425853225 hasRelatedWork W1974337415 @default.
• W2425853225 hasRelatedWork W2005919811 @default.
• W2425853225 hasRelatedWork W200707755 @default.
• W2425853225 hasRelatedWork W2010955060 @default.
• W2425853225 hasRelatedWork W2030099814 @default.
• W2425853225 hasRelatedWork W2041495736 @default.
• W2425853225 hasRelatedWork W2042258241 @default.
• W2425853225 hasRelatedWork W2082797941 @default.
• W2425853225 hasRelatedWork W2089273445 @default.
• W2425853225 hasRelatedWork W2091650241 @default.
• W2425853225 hasRelatedWork W2312814579 @default.
• W2425853225 hasRelatedWork W2315525852 @default.
• W2425853225 hasRelatedWork W2402657519 @default.
• W2425853225 hasRelatedWork W2403196040 @default.
• W2425853225 hasRelatedWork W2414091351 @default.
• W2425853225 hasRelatedWork W2414325521 @default.
• W2425853225 hasRelatedWork W2432278194 @default.
• W2425853225 hasRelatedWork W2436033863 @default.
• W2425853225 hasRelatedWork W88713852 @default.
• W2425853225 isParatext "false" @default.
• W2425853225 isRetracted "false" @default.
• W2425853225 magId "2425853225" @default.
• W2425853225 workType "article" @default.