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- W2428205544 abstract "A new method for studying the lipid-protein interactions in vitro is developed. It enables the study of the transporting activity of a protein toward a lipid ligand, including the case with an unknown lipid type. The method can be considered as a variant of partition three-phase chromatography with two stationary (donor and acceptor) phases and one mobile phase. The protein under study is dissolved in an aqueous mobile phase and induces a specific delivery of a lipid to the acceptor lipid layer. The transported lipid is identified in the Folch lipid extracts from the acceptor layer and aqueous phase. The secretory protein with M 45 kDa from the rat olfactory epithelium is shown to be a carrier of phosphatidylinositol 3,4,5-triphosphate. Our approach opens up new possibilities in the study of lipid-protein interactions in vitro and has a number of advantages over the methods now used for these purposes." @default.
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- W2428205544 date "2005-08-27" @default.
- W2428205544 modified "2023-09-23" @default.
- W2428205544 title "[A new method for studying the lipid transport in vitro: the protein with molecular weight 45-kDa from the rat olfactory epithelium--specific carrier of phosphatidylinositol 3,4,5-triphosphate]." @default.
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