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- W2428531014 abstract "HeLa cells contain four keratin-like proteins hav- ing molecular weights of 50,000 (IEF 31), 48,500 (IEF 36), 44,000 (IEF 44), and 43,500 (IEF 46), respectively. Mouse polyclonal an- tibodies prepared against two of these keratins (IEF 31 and 46) have been used in this study to identify human epidermal keratins with common antigenic determinants. Using a sensitive immu- noprecipitation procedure we show that the IEF 31 antibody crossreacts with three human acidic epidermal keratins, termed Kl, K2, and K3, having molecular weights of 44,000, 47,500, and 54,000, respectively. One of these keratins (Kl) comigrated with HeLa keratin IEF 44 and exhibited an identical one-dimensional peptide map. This protein is also abundant in basaliomas. In con- trast to these results, the IEF 46 antibody showed no crossreac- tivity with any of the human acidic or basic (35S)methionine-la- beled epidermal proteins. The lack of crossreactivity of this antibody was further confirmed by indirect immunofluorescence staining of cryostat sections from human split skin. These results emphasize both the similarity and diversity of antigenic deter- minants among HeLa and epidermal keratins." @default.
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- W2428531014 date "2016-01-01" @default.
- W2428531014 modified "2023-09-27" @default.
- W2428531014 title "Differential immunological crossrea antibodies with human epidermal k (epidermal proteins/two-dimensional gel electrophoresis/thin sections" @default.
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