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- W2428952610 abstract "Objective To investigate the differentiation of the rat bone marrow stromal cells (BMSCs) into neuron like cells which express glutamic acid decarboxylase (GAD) and choline acetyltransferase (ChAT). Methods BMSCs of rats were flushed out with DMEM from femurs marrow and separated, and then planted in plastic flask. After being expanded and purified, the BMSCs were differentiated into neuron-like cells. Neurofilament (NF-200), microassociate protein-2 (MAP-2), neuron-specific nuclear protein (NeuN), nestin, gial fibrillary acidic protein (GFAP), GAD and ChAT were detected by immunohisitochemistry. Results The induced BMSCs exhibited neuronal morphological characteristics, and the expressions of NF-200, MAP-2, NeuN and nestin were positive, but the expression of GFAP was negative. About (3 +/- 0.8)% of the induced BMSCs expressed GAD, and (5 +/- 0.3)% expressed ChAT. Conclusion BMSCs can differentiate into neuron like cells, and express GAD and ChAT partially." @default.
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- W2428952610 date "2004-02-01" @default.
- W2428952610 modified "2023-10-18" @default.
- W2428952610 title "Differentiation of rat bone marrow stromal cells into neuron like cells" @default.
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