Matches in SemOpenAlex for { <https://semopenalex.org/work/W2429087358> ?p ?o ?g. }
Showing items 1 to 72 of
72
with 100 items per page.
- W2429087358 endingPage "48" @default.
- W2429087358 startingPage "328" @default.
- W2429087358 abstract "Intracellular Ca2+ mobilization in the resting platelets undergoing Ca2+ influx and in the activated platelets stimulated by thrombin and phorbol ester (TPA) was investigated by measuring cytosolic Ca2+ concentration: [Ca2+]cyt (fura-2, aequorin), membrane-bound Ca2+ (chlortetracycline) and Ca2+ flux across the plasma membrane (45Ca2+ tracer). Some modified methods employed in this study concerning 45Ca2+ flux measurement and aequorin loading into platelets were very useful to elucidate the details of platelet Ca2+ mobilization in the combination with other Ca2+ assay methods. These combined and integrated assays on platelet Ca2+ showed some new interesting findings as follows; (1) The difference of the obtained values of [Ca2+]cyt and of the reaction pattern between fura-2 and aequorin method was responsible to the difference of the distribution of probe molecules in platelet. This was confirmed by the higher sensitivity of aequorin molecules to [Ca2+]cyt elevation induced by Ca2+ influx than by that of fura-2 molecules in thrombin- and TPA-activated platelets. (2) The membrane-bound Ca2+ release in activated platelets was found to be another intracellular Ca2+ movement distinct from organelle Ca2+ release and CTC molecules not to bind to the organelle membranes. (3) The plasma membrane Ca2+ pump was found to exist in both unstimulated platelets and stimulated platelets suggested by its specific time course of 45Ca2+ binding and [Ca2+]cyt increase in platelets. (4) There existed biphasic increase of [Ca2+]cyt in thrombin-stimulated platelets consisted of biphasic Ca2+ influx and biphasic intracellular Ca2+ release with alternating point at 10 seconds after activation. The initial changes seemed to be correlated with receptor operated Ca2+ channel and receptor-linked phospholipase C activation respectively, while both second phase increase seemed to be correlated with the plasma membrane phospholipid metabolisms evoked by phospholipase A2 activation." @default.
- W2429087358 created "2016-06-24" @default.
- W2429087358 creator A5016581477 @default.
- W2429087358 date "1989-05-01" @default.
- W2429087358 modified "2023-09-23" @default.
- W2429087358 title "[Significance of multiple analysis of platelet Ca2+ mobilization by using some available methods measuring cytosolic Ca2+ concentration, membrane-bound Ca2+ and Ca2+ flux across the plasma membrane]." @default.
- W2429087358 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2767617" @default.
- W2429087358 hasPublicationYear "1989" @default.
- W2429087358 type Work @default.
- W2429087358 sameAs 2429087358 @default.
- W2429087358 citedByCount "0" @default.
- W2429087358 crossrefType "journal-article" @default.
- W2429087358 hasAuthorship W2429087358A5016581477 @default.
- W2429087358 hasConcept C12554922 @default.
- W2429087358 hasConcept C168240541 @default.
- W2429087358 hasConcept C178790620 @default.
- W2429087358 hasConcept C181199279 @default.
- W2429087358 hasConcept C185592680 @default.
- W2429087358 hasConcept C203014093 @default.
- W2429087358 hasConcept C23265538 @default.
- W2429087358 hasConcept C2777292125 @default.
- W2429087358 hasConcept C2778470358 @default.
- W2429087358 hasConcept C3018697912 @default.
- W2429087358 hasConcept C41625074 @default.
- W2429087358 hasConcept C51705589 @default.
- W2429087358 hasConcept C519063684 @default.
- W2429087358 hasConcept C55493867 @default.
- W2429087358 hasConcept C79879829 @default.
- W2429087358 hasConcept C80933277 @default.
- W2429087358 hasConcept C86803240 @default.
- W2429087358 hasConcept C89560881 @default.
- W2429087358 hasConcept C98539663 @default.
- W2429087358 hasConceptScore W2429087358C12554922 @default.
- W2429087358 hasConceptScore W2429087358C168240541 @default.
- W2429087358 hasConceptScore W2429087358C178790620 @default.
- W2429087358 hasConceptScore W2429087358C181199279 @default.
- W2429087358 hasConceptScore W2429087358C185592680 @default.
- W2429087358 hasConceptScore W2429087358C203014093 @default.
- W2429087358 hasConceptScore W2429087358C23265538 @default.
- W2429087358 hasConceptScore W2429087358C2777292125 @default.
- W2429087358 hasConceptScore W2429087358C2778470358 @default.
- W2429087358 hasConceptScore W2429087358C3018697912 @default.
- W2429087358 hasConceptScore W2429087358C41625074 @default.
- W2429087358 hasConceptScore W2429087358C51705589 @default.
- W2429087358 hasConceptScore W2429087358C519063684 @default.
- W2429087358 hasConceptScore W2429087358C55493867 @default.
- W2429087358 hasConceptScore W2429087358C79879829 @default.
- W2429087358 hasConceptScore W2429087358C80933277 @default.
- W2429087358 hasConceptScore W2429087358C86803240 @default.
- W2429087358 hasConceptScore W2429087358C89560881 @default.
- W2429087358 hasConceptScore W2429087358C98539663 @default.
- W2429087358 hasIssue "3" @default.
- W2429087358 hasLocation W24290873581 @default.
- W2429087358 hasOpenAccess W2429087358 @default.
- W2429087358 hasPrimaryLocation W24290873581 @default.
- W2429087358 hasRelatedWork W1981715101 @default.
- W2429087358 hasRelatedWork W2009020831 @default.
- W2429087358 hasRelatedWork W2032864168 @default.
- W2429087358 hasRelatedWork W2085260413 @default.
- W2429087358 hasRelatedWork W2090526665 @default.
- W2429087358 hasRelatedWork W2362336422 @default.
- W2429087358 hasRelatedWork W2401678231 @default.
- W2429087358 hasRelatedWork W2414646060 @default.
- W2429087358 hasRelatedWork W2429087358 @default.
- W2429087358 hasRelatedWork W4256484403 @default.
- W2429087358 hasVolume "64" @default.
- W2429087358 isParatext "false" @default.
- W2429087358 isRetracted "false" @default.
- W2429087358 magId "2429087358" @default.
- W2429087358 workType "article" @default.