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• W2429121879 startingPage "251" @default.
• W2429121879 abstract "Transferring genetic molecules into the peripheral sensory nervous system to manipulate nociceptive pathophysiology is a powerful approach for experimental modulation of sensory signaling and potentially for translation into therapy for chronic pain. This can be efficiently achieved by the use of recombinant adeno-associated virus (rAAV) in conjunction with nociceptor-specific regulatory transgene cassettes. Among different routes of delivery, direct injection into the dorsal root ganglia (DRGs) offers the most efficient AAV-mediated gene transfer selectively into the peripheral sensory nervous system. Here, we briefly discuss the advantages and applications of intraganglionic microinjection, and then provide a detailed approach for DRG injection, including a list of the necessary materials and description of a method for performing DRG microinjection experiments. We also discuss our experience with several adeno-associated virus (AAV) options for in vivo transgene expression in DRG neurons." @default.
• W2429121879 created "2016-06-24" @default.
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• W2429121879 date "2016-01-01" @default.
• W2429121879 modified "2023-10-03" @default.
• W2429121879 title "AAV-Mediated Gene Transfer to Dorsal Root Ganglion" @default.
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• W2429121879 doi "https://doi.org/10.1007/978-1-4939-3271-9_18" @default.
• W2429121879 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5459312" @default.
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• W2429121879 hasPublicationYear "2016" @default.
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