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- W2429646565 abstract "OBJECTIVE To investigate the effects of the cell wall of Streptococcus mutans on the expression of Toll-like receptor 4 (TLR4), IL-6 and IL-8 in EAhy926 cells (The human endothelial hybridoma of human umbilical vein endothelial cells (HUVEC) and the human epithelial cell line A549, characterized by endothelial phenotype and biology). METHODS EAhy926 cells were treated with different concentrations of the cell wall of Streptococcus mutans. The proliferation of EAhy926 cells was determined by MTT assay. The mRNA expression of TLR4, IL-6 and IL-8 in EAhy926 cells was detected by reverse transcription polymerase chain reaction (RT-PCR). TLR4 was analyzed by flow cytometry. The production of IL-6 and IL-8 in the cultured supernatants was measured by biochemical method and ELISA respectively. TLR4 blocking assay was used to investigate the relationship between IL-6, IL-8 and TLR4 mRNA expression. RESULTS The proliferation of EAhy926 cells was significantly enhanced by the cell wall of Streptococcus mutans at 6 h (P<0.05), and inhibited after 12 h (P<0.05), both time- and dose-dependent. The expression of mRNA and protein for TLR4 in EAhy926 cells was increased after stimulated by the cell wall of Streptococcus mutans, peaking at 16 h (P<0.05), and then decreased. The expression of IL-6 and IL-8 mRNA was significantly induced when exposed to the cell wall, reaching the maximal level at 16 h and 24 h, respectively (P<0.05). Meanwhile, the cell wall of Streptococcus mutans induced the production of IL-6 and IL-8 (P<0.05). The expression of IL-6 and IL-8 mRNA in EAhy926 cells was blocked by anti-human TLR4 antibodies significantly. CONCLUSION The cell wall of Streptococcus mutans upregulated the expression of TLR4 and induced the production of inflammatory cytokines IL-6 and IL-8, indicating that the expression of TLR4 of EAhy926 cells may elicit a TLR4-mediated innate immune response and contribute to production of inflammatory cytokines IL-6 and IL-8." @default.
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- W2429646565 date "2010-02-01" @default.
- W2429646565 modified "2023-09-26" @default.
- W2429646565 title "[Effects of the cell wall of Streptococcus mutans on the expression of TLR4, IL-6 and IL-8 by EAhy926 cells]." @default.
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