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• W2430370070 abstract "High-resolution imaging of the membranous intermediates and cytoskeletal arrays involved in the assembly of a new cell wall during plant cytokinesis requires state-of-the-art electron microscopy techniques. The combination of cryofixation/freeze-substitution methods with electron tomography (ET) has revealed amazing structural details of this unique cellular process. This chapter deals with the main steps associated with these imaging techniques: selection of samples suitable for studying plant cytokinesis, sample preparation by high-pressure freezing/freeze substitution, and ET of plastic sections. In addition, immunogold approaches for the identification of proteins and polysaccharides during cell wall assembly are discussed." @default.
• W2430370070 created "2016-06-24" @default.
• W2430370070 creator A5021534135 @default.
• W2430370070 date "2010-12-13" @default.
• W2430370070 modified "2023-10-16" @default.
• W2430370070 title "Electron Tomography and Immunogold Labelling as Tools to Analyse De Novo Assembly of Plant Cell Walls" @default.
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• W2430370070 doi "https://doi.org/10.1007/978-1-61779-008-9_9" @default.
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