Matches in SemOpenAlex for { <https://semopenalex.org/work/W2430441117> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W2430441117 endingPage "333" @default.
- W2430441117 startingPage "329" @default.
- W2430441117 abstract "Trichoderma koningii G-39 produced xylanases in submerged culture using oat spelt xylan or crystalline cellulose, Avicel, as the sole carbon source. A low-Mr xylanase was purified from the culture filtrate by ion-exchange chromatography on SP-Trisacryl-M and gel filtration on Fractogel TSK HW-50F. It was homogeneous on SDS/PAGE and isoelectric focusing. A typical procedure provided about 11-fold purification with 4.5% protein yield and 50% activity recovery. The purified enzyme has an Mr value of about 21,500 and a pI of 8.9. Its specific activity was 6100 units/mg of protein, with optimal activity towards 0.5% xylan at about pH 5.5 and 60 degrees C. The purified enzyme had no activity against CM-cellulose with a degree of substitution of 0.63. It also showed no beta-xylosidase activity. The Km and Vmax. values, as determined with the soluble fraction of oat spelt xylan as substrate, were 0.70 mg/ml and 1.85 x 10(6) mumol/min per mg of enzyme respectively. Hg2+ (1 mM) and SDS (10 mM) completely inhibited xylanase activity, whereas Ca2+ showed no significant effect on the enzyme activity at 1 mM, but gave 80% inhibition at 10 mM. The enzyme contained about 4.4% carbohydrate and showed an immunological relationship to a cellobiohydrolase from the same fungal strain." @default.
- W2430441117 created "2016-06-24" @default.
- W2430441117 creator A5005456952 @default.
- W2430441117 creator A5023325607 @default.
- W2430441117 creator A5032783449 @default.
- W2430441117 date "1991-09-01" @default.
- W2430441117 modified "2023-09-25" @default.
- W2430441117 title "Purification and characterization of an endoxylanase from Trichoderma koningii G-39" @default.
- W2430441117 cites W1490966811 @default.
- W2430441117 cites W1544083863 @default.
- W2430441117 cites W1863250985 @default.
- W2430441117 cites W1997180556 @default.
- W2430441117 cites W2018289835 @default.
- W2430441117 cites W2100837269 @default.
- W2430441117 cites W2168553434 @default.
- W2430441117 cites W299204690 @default.
- W2430441117 doi "https://doi.org/10.1042/bj2780329" @default.
- W2430441117 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1151344" @default.
- W2430441117 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1910330" @default.
- W2430441117 hasPublicationYear "1991" @default.
- W2430441117 type Work @default.
- W2430441117 sameAs 2430441117 @default.
- W2430441117 citedByCount "40" @default.
- W2430441117 countsByYear W24304411172012 @default.
- W2430441117 countsByYear W24304411172013 @default.
- W2430441117 countsByYear W24304411172014 @default.
- W2430441117 countsByYear W24304411172015 @default.
- W2430441117 countsByYear W24304411172017 @default.
- W2430441117 countsByYear W24304411172020 @default.
- W2430441117 countsByYear W24304411172021 @default.
- W2430441117 crossrefType "journal-article" @default.
- W2430441117 hasAuthorship W2430441117A5005456952 @default.
- W2430441117 hasAuthorship W2430441117A5023325607 @default.
- W2430441117 hasAuthorship W2430441117A5032783449 @default.
- W2430441117 hasBestOaLocation W24304411172 @default.
- W2430441117 hasConcept C100817775 @default.
- W2430441117 hasConcept C116174643 @default.
- W2430441117 hasConcept C141603559 @default.
- W2430441117 hasConcept C181199279 @default.
- W2430441117 hasConcept C185592680 @default.
- W2430441117 hasConcept C18903297 @default.
- W2430441117 hasConcept C193363816 @default.
- W2430441117 hasConcept C198684833 @default.
- W2430441117 hasConcept C2775859485 @default.
- W2430441117 hasConcept C2776125036 @default.
- W2430441117 hasConcept C2777289219 @default.
- W2430441117 hasConcept C2779251873 @default.
- W2430441117 hasConcept C28212737 @default.
- W2430441117 hasConcept C43617362 @default.
- W2430441117 hasConcept C50476208 @default.
- W2430441117 hasConcept C55493867 @default.
- W2430441117 hasConcept C86803240 @default.
- W2430441117 hasConcept C96294017 @default.
- W2430441117 hasConceptScore W2430441117C100817775 @default.
- W2430441117 hasConceptScore W2430441117C116174643 @default.
- W2430441117 hasConceptScore W2430441117C141603559 @default.
- W2430441117 hasConceptScore W2430441117C181199279 @default.
- W2430441117 hasConceptScore W2430441117C185592680 @default.
- W2430441117 hasConceptScore W2430441117C18903297 @default.
- W2430441117 hasConceptScore W2430441117C193363816 @default.
- W2430441117 hasConceptScore W2430441117C198684833 @default.
- W2430441117 hasConceptScore W2430441117C2775859485 @default.
- W2430441117 hasConceptScore W2430441117C2776125036 @default.
- W2430441117 hasConceptScore W2430441117C2777289219 @default.
- W2430441117 hasConceptScore W2430441117C2779251873 @default.
- W2430441117 hasConceptScore W2430441117C28212737 @default.
- W2430441117 hasConceptScore W2430441117C43617362 @default.
- W2430441117 hasConceptScore W2430441117C50476208 @default.
- W2430441117 hasConceptScore W2430441117C55493867 @default.
- W2430441117 hasConceptScore W2430441117C86803240 @default.
- W2430441117 hasConceptScore W2430441117C96294017 @default.
- W2430441117 hasIssue "2" @default.
- W2430441117 hasLocation W24304411171 @default.
- W2430441117 hasLocation W24304411172 @default.
- W2430441117 hasLocation W24304411173 @default.
- W2430441117 hasLocation W24304411174 @default.
- W2430441117 hasOpenAccess W2430441117 @default.
- W2430441117 hasPrimaryLocation W24304411171 @default.
- W2430441117 hasRelatedWork W1635294277 @default.
- W2430441117 hasRelatedWork W2007947065 @default.
- W2430441117 hasRelatedWork W2015619748 @default.
- W2430441117 hasRelatedWork W2023471803 @default.
- W2430441117 hasRelatedWork W2041275422 @default.
- W2430441117 hasRelatedWork W2045151858 @default.
- W2430441117 hasRelatedWork W2087373518 @default.
- W2430441117 hasRelatedWork W2090264190 @default.
- W2430441117 hasRelatedWork W2430441117 @default.
- W2430441117 hasRelatedWork W338539550 @default.
- W2430441117 hasVolume "278" @default.
- W2430441117 isParatext "false" @default.
- W2430441117 isRetracted "false" @default.
- W2430441117 magId "2430441117" @default.
- W2430441117 workType "article" @default.