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- W2430555826 abstract "Genome-wide identification of transcription factor binding sites with the ChIP-seq method is an extremely important scientific endeavor - one that should ideally be performed for every transcription factor in as many cell types as possible. A major hurdle on the way to this goal is the necessity for a specific, ChIP-grade antibody for each transcription factor of interest, which is often not available. Here, we describe CETCh-seq, a recently published method utilizing genome engineering with the CRISPR/Cas9 system to circumvent the need for a specific antibody. Using the CETCh-seq method, targeted genomic editing results in an epitope-tagged transcription factor, which is recognized by a well-characterized, standard antibody, efficacious for ChIP-seq. We have used CETCh-seq in human cancer cell lines as well as mouse embryonic stem cells. We find that roughly 60% of transcription factors tagged using CETCh-seq produce a high quality ChIP-seq map, a significant improvement over traditional antibody-based methods." @default.
- W2430555826 created "2016-06-24" @default.
- W2430555826 creator A5028295882 @default.
- W2430555826 creator A5050873069 @default.
- W2430555826 creator A5064134736 @default.
- W2430555826 date "2016-06-17" @default.
- W2430555826 modified "2023-09-24" @default.
- W2430555826 title "Every transcription factor deserves its map: Scaling up epitope tagging of proteins to bypass antibody problems" @default.
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- W2430555826 doi "https://doi.org/10.1002/bies.201600028" @default.
- W2430555826 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27311628" @default.
- W2430555826 hasPublicationYear "2016" @default.
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