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- W2431730211 abstract "Aponogeton madagascariensis is the only aquatic plant which forms perforations in its leaves through a process known as programmed cell death (PCD). Although PCD can be studied in vivo, isolated protoplasts maintain many of the same physiological properties of the intact plant and, due to their accessibility and reduced complexity, make ideal research tools to investigate plant processes including PCD. The conditions for the isolation of protoplasts from the lace plant were examined. Several factors including leaf age, carbohydrate source, and enzyme incubation time all significantly influenced protoplast yield and viability from lace plant tissue. Viable protoplasts were successfully isolated from leaf tissue approximately 1 month in age, using an enzyme mixture comprised of 2% w/v cellulase onozuka R10 and 0.5% pectolyase Y-23 dissolved in 0.005 M MES and 0.6 M sorbitol, pH 5.5. Approximately 60.35 ± 1.08 x 10 5 protoplasts/g fresh weight, with a viability of 92.75 ± 2.25%, were obtained from 4 hour isolations in the dark. Following successful isolation, these lace plant protoplasts can be used as an excellent model system for the study of environmentally induced PCD and can provide the first ever opportunity to compare this form of PCD with its developmentally regulated counterpart within one species of plant." @default.
- W2431730211 created "2016-06-24" @default.
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- W2431730211 date "2010-01-01" @default.
- W2431730211 modified "2023-09-24" @default.
- W2431730211 title "Isolation of Leaf Protoplasts from the Submerged Aquatic Monocot Aponogeton madagascariensis Christina E. N. LordArunika H. L. A. N. Gunawardena" @default.
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