Matches in SemOpenAlex for { <https://semopenalex.org/work/W243191262> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W243191262 abstract "The ability to strongly and sequence-specifically attach modifications such as fluorophores and haptens to double-stranded DNA is critical to a variety of single-molecule experiments. We present a practical guide to labeling internal sequences of double-stranded (ds) DNA molecules for single-molecule experiments. Since single-molecule experiments are diverse in their requirements, we focus on six approaches and consider the merits and drawbacks of each. By presenting a set of criteria relevant to single-molecule experiments (e.g. labeling yield, compatibility with cofactors), the guide provides a simple reference for selecting a labeling approach for given experimental constraints. We study one of these approaches, Peptide Nucleic Acids (PNAs), in detail. Slide stretching is used to optimize DNA-PNA binding and microfluidic trapping and stretching in a cross-slot demonstrates fluorescence-based sequence detection using PNAs on a long, roughly 50 kb, untethered DNA molecule. Optical tweezer experiments on DNA-bound PNAs further demonstrate that PNAs are versatile and robust sequence-specific tethers. Lastly, we assess the feasibility of studying DNA-protein interactions between stained DNA and fluorescently-labeled protein at the single-molecule level in the microfluidic cross-slot using the motor protein SpoIIIE." @default.
- W243191262 created "2016-06-24" @default.
- W243191262 creator A5048524315 @default.
- W243191262 date "2011-01-01" @default.
- W243191262 modified "2023-09-27" @default.
- W243191262 title "Tools for Single-Molecule Sequence Detection and Manipulation" @default.
- W243191262 hasPublicationYear "2011" @default.
- W243191262 type Work @default.
- W243191262 sameAs 243191262 @default.
- W243191262 citedByCount "0" @default.
- W243191262 crossrefType "journal-article" @default.
- W243191262 hasAuthorship W243191262A5048524315 @default.
- W243191262 hasConcept C121332964 @default.
- W243191262 hasConcept C12554922 @default.
- W243191262 hasConcept C171250308 @default.
- W243191262 hasConcept C178790620 @default.
- W243191262 hasConcept C185592680 @default.
- W243191262 hasConcept C186060115 @default.
- W243191262 hasConcept C191961535 @default.
- W243191262 hasConcept C192562407 @default.
- W243191262 hasConcept C199191878 @default.
- W243191262 hasConcept C24107716 @default.
- W243191262 hasConcept C2778112365 @default.
- W243191262 hasConcept C32909587 @default.
- W243191262 hasConcept C49853544 @default.
- W243191262 hasConcept C51679486 @default.
- W243191262 hasConcept C552990157 @default.
- W243191262 hasConcept C55493867 @default.
- W243191262 hasConcept C62520636 @default.
- W243191262 hasConcept C70721500 @default.
- W243191262 hasConcept C8673954 @default.
- W243191262 hasConcept C86803240 @default.
- W243191262 hasConcept C91881484 @default.
- W243191262 hasConceptScore W243191262C121332964 @default.
- W243191262 hasConceptScore W243191262C12554922 @default.
- W243191262 hasConceptScore W243191262C171250308 @default.
- W243191262 hasConceptScore W243191262C178790620 @default.
- W243191262 hasConceptScore W243191262C185592680 @default.
- W243191262 hasConceptScore W243191262C186060115 @default.
- W243191262 hasConceptScore W243191262C191961535 @default.
- W243191262 hasConceptScore W243191262C192562407 @default.
- W243191262 hasConceptScore W243191262C199191878 @default.
- W243191262 hasConceptScore W243191262C24107716 @default.
- W243191262 hasConceptScore W243191262C2778112365 @default.
- W243191262 hasConceptScore W243191262C32909587 @default.
- W243191262 hasConceptScore W243191262C49853544 @default.
- W243191262 hasConceptScore W243191262C51679486 @default.
- W243191262 hasConceptScore W243191262C552990157 @default.
- W243191262 hasConceptScore W243191262C55493867 @default.
- W243191262 hasConceptScore W243191262C62520636 @default.
- W243191262 hasConceptScore W243191262C70721500 @default.
- W243191262 hasConceptScore W243191262C8673954 @default.
- W243191262 hasConceptScore W243191262C86803240 @default.
- W243191262 hasConceptScore W243191262C91881484 @default.
- W243191262 hasLocation W2431912621 @default.
- W243191262 hasOpenAccess W243191262 @default.
- W243191262 hasPrimaryLocation W2431912621 @default.
- W243191262 hasRelatedWork W1993898634 @default.
- W243191262 hasRelatedWork W1997628368 @default.
- W243191262 hasRelatedWork W2005394279 @default.
- W243191262 hasRelatedWork W2011304608 @default.
- W243191262 hasRelatedWork W2045517527 @default.
- W243191262 hasRelatedWork W2051970551 @default.
- W243191262 hasRelatedWork W2144506694 @default.
- W243191262 hasRelatedWork W2147806005 @default.
- W243191262 hasRelatedWork W2163202955 @default.
- W243191262 hasRelatedWork W2332817766 @default.
- W243191262 hasRelatedWork W2597778366 @default.
- W243191262 hasRelatedWork W2742195959 @default.
- W243191262 hasRelatedWork W2810473937 @default.
- W243191262 hasRelatedWork W2908370653 @default.
- W243191262 hasRelatedWork W2989448362 @default.
- W243191262 hasRelatedWork W3201850835 @default.
- W243191262 hasRelatedWork W1564640930 @default.
- W243191262 hasRelatedWork W1981131974 @default.
- W243191262 hasRelatedWork W2929230320 @default.
- W243191262 hasRelatedWork W3075362793 @default.
- W243191262 isParatext "false" @default.
- W243191262 isRetracted "false" @default.
- W243191262 magId "243191262" @default.
- W243191262 workType "article" @default.