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• W2431923069 abstract "To elucidate the role of the molecular structure of adhesive proteins in an endothelialization of synthetic vascular prosthesis in vitro, a recombinant fibronectin-like engineered adhesion factor (FP) constructed from the specific Arg-Gly-Asp cell adhesion repeats was assayed as adhesive substrate to culture human saphenous vein endothelial cells on ePTFE. ePTFE samples (1 cm 2 ) inserted into cell culture chambers were coated by incubation with increasing amounts of FP (up to 100 μg/cm 2 ) prior to cell seeding. At 24 hours after low density cell seeding and 20 μg/ml/cm 2 FP concentration, the number of adhered cells reached a plateau and the adhered cells did not proliferate up to 6 days of culture. At 24 hours after high density seeding (10 5 cells/cm 2 ), the number of adhered cells was significantly higher on ePTFE with preadsorbed FP than on fibronectin coated PTFE. About 55% of the initially adhered cells survived up to 7 days on FP, whereas cell debris and free nuclei were predominant on fibronectin coated PTFE. In the investigated model the engineered RGD polymer potentialized a short-term adhesion of vascular endothelial cells to PTFE, nevertheless it did not ensure proliferation and long-term survival of these cells." @default.
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• W2431923069 date "1994-02-01" @default.
• W2431923069 modified "2023-10-17" @default.
• W2431923069 title "Human Vascular Endothelial Cells on Expanded Ptfe Precoated with An Engineered Protein Adhesion Factor" @default.
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• W2431923069 doi "https://doi.org/10.1177/039139889401700209" @default.
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