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- W243232493 abstract "TCDD and related polyhalogenated aromatic hydrocarbons cause toxicity which involves dysregulation of cellular homeostasis. They also induce cytochrome P450s via the cytosolic Ah receptor, markedly increasing 7-ethoxyresorufin O-deethylase (7-EROD) and aryl hydrocarbon hydroxylase (AHH) in many species, including CE. The authors found that TCDD also induced NADPH-dependent P450-mediated metabolism of arachidonic acid (AA) in CEL. They isolated two TCDD inducible P450s from CEL microsomes. One, (TCDD{sub AA}, 55 kDa) in reconstituted systems effectively catalyzed AA metabolism but not 7-EROD or AHH; mean turnover numbers for AA metabolism, 7-EROD and AHH were 35, 0.5, and 0.2, respectively. This P450 was a selective epoxygenase. In contrast, another P450 catalyzed 7-EROD and AHH much more effectively than AA metabolism; mean turnover numbers for AA metabolism, 7-EROD and AHH were 0.8, 36 and 10, respectively. The N-terminal sequences for TCDD{sub AA} and TCDD{sub AHH} had about 80% homology. The N-terminal sequence, catalytic activity, molecular weight and chromatographic behavior of TCDD{sub AA} were identical to those of a P-450 purified from {beta}-naphthoflavone ({beta}NF) induced CEL microsomes, indicating that TCDD and {beta}NF induced the same selective AA epoxygenase. Polyclonal antibodies against the P450s showed partial cross reactivity on Western blots. Their findings establish a biochemical basismore » for a link between TCDD induction of P450 and its alterations in cellular homeostasis in the CE.« less" @default.
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- W243232493 date "1992-02-26" @default.
- W243232493 modified "2023-09-24" @default.
- W243232493 title "Purification and characterization of two inducible P450s from 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) treated chick embryo liver (CEL) microsomes" @default.
- W243232493 hasPublicationYear "1992" @default.
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