FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2433272693> ?p ?o ?g. }

• W2433272693 endingPage "1109" @default.
• W2433272693 startingPage "1100" @default.
• W2433272693 abstract "Mammalian cell line development requires streamlined methodologies that will reduce both the cost and time to identify candidate cell lines. Improvements in site-specific genomic editing techniques can result in flexible, predictable, and robust cell line engineering. However, an outstanding question in the field is the specific site of integration. Here, we seek to identify productive loci within the human genome that will result in stable, high expression of heterologous DNA. Using an unbiased, random integration approach and a green fluorescent reporter construct, we identify ten single-integrant, recombinant human cell lines that exhibit stable, high-level expression. From these cell lines, eight unique corresponding integration loci were identified. These loci are concentrated in non-protein coding regions or intronic regions of protein coding genes. Expression mapping of the surrounding genes reveals minimal disruption of endogenous gene expression. Finally, we demonstrate that targeted de novo integration at one of the identified loci, the 12(th) exon-intron region of the GRIK1 gene on chromosome 21, results in superior expression and stability compared to the standard, illegitimate integration approach at levels approaching 4-fold. The information identified here along with recent advances in site-specific genomic editing techniques can lead to expedited cell line development." @default.
• W2433272693 created "2016-06-24" @default.
• W2433272693 creator A5023917549 @default.
• W2433272693 creator A5030477224 @default.
• W2433272693 creator A5035405793 @default.
• W2433272693 creator A5043427732 @default.
• W2433272693 creator A5044207810 @default.
• W2433272693 date "2016-06-30" @default.
• W2433272693 modified "2023-10-16" @default.
• W2433272693 title "Identifying and retargeting transcriptional hot spots in the human genome" @default.
• W2433272693 cites W1518634270 @default.
• W2433272693 cites W1582042869 @default.
• W2433272693 cites W1637867377 @default.
• W2433272693 cites W1924593714 @default.
• W2433272693 cites W1934845822 @default.
• W2433272693 cites W1968741040 @default.
• W2433272693 cites W1972148441 @default.
• W2433272693 cites W1977955083 @default.
• W2433272693 cites W1980869599 @default.
• W2433272693 cites W1981083117 @default.
• W2433272693 cites W1984820965 @default.
• W2433272693 cites W1984865246 @default.
• W2433272693 cites W1985610764 @default.
• W2433272693 cites W1989119577 @default.
• W2433272693 cites W1999708510 @default.
• W2433272693 cites W2000206290 @default.
• W2433272693 cites W2002387807 @default.
• W2433272693 cites W2003171404 @default.
• W2433272693 cites W2006552859 @default.
• W2433272693 cites W2006765654 @default.
• W2433272693 cites W2013550946 @default.
• W2433272693 cites W2021099440 @default.
• W2433272693 cites W2025074364 @default.
• W2433272693 cites W2029197798 @default.
• W2433272693 cites W2033767465 @default.
• W2433272693 cites W2035798715 @default.
• W2433272693 cites W2036093904 @default.
• W2433272693 cites W2041801904 @default.
• W2433272693 cites W2045618741 @default.
• W2433272693 cites W2046156611 @default.
• W2433272693 cites W2049582819 @default.
• W2433272693 cites W2049974202 @default.
• W2433272693 cites W2052407981 @default.
• W2433272693 cites W2075889739 @default.
• W2433272693 cites W2079554839 @default.
• W2433272693 cites W2080665724 @default.
• W2433272693 cites W2081150787 @default.
• W2433272693 cites W2085755265 @default.
• W2433272693 cites W2088032964 @default.
• W2433272693 cites W2090098862 @default.
• W2433272693 cites W2091852461 @default.
• W2433272693 cites W2093343938 @default.
• W2433272693 cites W2097399693 @default.
• W2433272693 cites W2105764211 @default.
• W2433272693 cites W2106898941 @default.
• W2433272693 cites W2113000430 @default.
• W2433272693 cites W2118540273 @default.
• W2433272693 cites W2119479524 @default.
• W2433272693 cites W2121074166 @default.
• W2433272693 cites W2124606386 @default.
• W2433272693 cites W2127582347 @default.
• W2433272693 cites W2130529538 @default.
• W2433272693 cites W2143549462 @default.
• W2433272693 cites W2151316444 @default.
• W2433272693 cites W2159213603 @default.
• W2433272693 cites W2159686532 @default.
• W2433272693 cites W2159925483 @default.
• W2433272693 cites W2161733245 @default.
• W2433272693 cites W2168619204 @default.
• W2433272693 cites W2230199130 @default.
• W2433272693 cites W2354242643 @default.
• W2433272693 doi "https://doi.org/10.1002/biot.201600015" @default.
• W2433272693 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27311394" @default.
• W2433272693 hasPublicationYear "2016" @default.
• W2433272693 type Work @default.
• W2433272693 sameAs 2433272693 @default.
• W2433272693 citedByCount "18" @default.
• W2433272693 countsByYear W24332726932018 @default.
• W2433272693 countsByYear W24332726932019 @default.
• W2433272693 countsByYear W24332726932020 @default.
• W2433272693 countsByYear W24332726932021 @default.
• W2433272693 countsByYear W24332726932022 @default.
• W2433272693 countsByYear W24332726932023 @default.
• W2433272693 crossrefType "journal-article" @default.
• W2433272693 hasAuthorship W2433272693A5023917549 @default.
• W2433272693 hasAuthorship W2433272693A5030477224 @default.
• W2433272693 hasAuthorship W2433272693A5035405793 @default.
• W2433272693 hasAuthorship W2433272693A5043427732 @default.
• W2433272693 hasAuthorship W2433272693A5044207810 @default.
• W2433272693 hasBestOaLocation W24332726931 @default.
• W2433272693 hasConcept C104317684 @default.
• W2433272693 hasConcept C141231307 @default.
• W2433272693 hasConcept C144501496 @default.
• W2433272693 hasConcept C197077220 @default.
• W2433272693 hasConcept C2780575108 @default.
• W2433272693 hasConcept C31972630 @default.
• W2433272693 hasConcept C41008148 @default.
• W2433272693 hasConcept C54355233 @default.

• next